Microchimica Acta

, 185:417 | Cite as

A 3-dimensional C/CeO2 hollow nanostructure framework as a peroxidase mimetic, and its application to the colorimetric determination of hydrogen peroxide

  • Nan Wang
  • Jizhou DuanEmail author
  • Weijie Shi
  • Xiaofan Zhai
  • Fang Guan
  • Lihui Yang
  • Baorong Hou
Original Paper


Various 3-dimensional C/CeO2 hollow nanostructure frameworks (3D C/CeO2 HNFs) were synthesized by using a polymer blowing process that is accelerated by adding a certain amount of cerium nitrate. Polyvinylpyrrolidone was used as the polymer. The resulting HNFs were characterized by scanning electron microscopy, energy dispersive spectrometry, X-ray diffraction, and X-ray photoelectron spectroscopy. The HNFs possess a large specific surface area, and the CeO2 nanocrystals consist of a single phase. The HNFs display intrinsic peroxidase-like activity and can catalyze the oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine in the presence of H2O2 to produce a blue product. The method was applied to the quantification of H2O2 with a 5.2 nM detection limit. The analytical range is from 10 nM to 1 μM.

Graphical abstract

Schematic of the preparation of a 3-dimensional C/CeO2 hollow nanostructure framework by a polyvinylpyrrolidone-blowing process accelerated by Ce(NO3)3. They were applied to H2O2 detection by catalyzing the oxidation of peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to the oxidized 3,3′,5,5′-tetramethylbenzidine (oxTMB) to produce blue-color reaction.


Microporous structure Photometry Catalytic activity H2O2 detection 



The present work was supported by the National Key Basic Research Project (No. 2014CB643304) and the National Natural Science Foundation of China (No. 41576080 and No. 41706080).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2957_MOESM1_ESM.doc (141 kb)
ESM 1 (DOC 141 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Nan Wang
    • 1
    • 2
    • 3
    • 4
  • Jizhou Duan
    • 1
    • 3
    • 4
    Email author
  • Weijie Shi
    • 5
  • Xiaofan Zhai
    • 1
    • 3
    • 4
  • Fang Guan
    • 1
    • 3
    • 4
  • Lihui Yang
    • 1
    • 3
    • 4
  • Baorong Hou
    • 1
    • 3
    • 4
  1. 1.Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Open Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao)QingdaoChina
  4. 4.Center for Ocean Mega-ScienceChinese Academy of SciencesQingdaoPeople’s Republic of China
  5. 5.College of Chemistry and Material ScienceShandong Agricultural UniversityTaianPeople’s Republic of China

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