Colorimetric determination of sialic acid based on boronic acid-mediated aggregation of gold nanoparticles
The authors describe a rapid, sensitive and selective colorimetric assay for sialic acid (SA) based on the use of gold nanoparticles (AuNPs) modified with 3-aminophenylboronic acid (3-APBA) which acts as the recognition probe for SA. 3-APBA contains amino groups and boronic acid groups through which it can assemble on citrate stabilized AuNPs. It reacts with the cis-diol groups of SA by reversible formation of a cyclic boronate ester in slightly acidic buffer. Detection involves the sequential addition of AuNPs, phosphate buffer, 3-APBA and SA in a tube, vortex mixing, acquisition of photographic images or absorption spectra, and calculation of the result. The method is simple, rapid, and does not require cumbersome steps such as the preparation of stable boronic acid functionalized AuNPs as used in colorimetric sensing of saccharides. Under the optimum conditions, the ratio of absorbances at 700 and 520 nm increases linearly in the 0.15–1.00 mM SA concentrations range, and the detection limit is 60 µM. This is comparable to the detection limit obtained in other colorimetric assays reported. Acceptable intra- and inter-day precisions of three SA concentrations (0.50, 1.00 and 2.00 mM) ranged from 1.9−4.2% and 4.2−6.4%, respectively. The efficacy of the method was demonstrated by analyzing simulated human saliva which gave recoveries ranging from 98.7−106.0%.
KeywordsGold nanoparticles 3-Aminophenyl boronic acid Sialic acid Colorimetry Aggregation Simulated human saliva
This research work was funded and supported by the Graduate studies grant under the International Student Scholarship, Faculty of Science, Prince of Songkla University. The Department of Chemistry and the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Office of the Higher Education Commission, Ministry of Education (OHEC), Prince of Songkla University are highly appreciated. Jayeoye Titilope J would like to acknowledge and appreciate the support of the Federal University Ndufu Alike Ikwo, Abakaliki, Ebonyi state, Nigeria, for the approval of study leave to study in Thailand.
Compliance with ethical standards
The author(s) declare that they have no competing interest.
- 7.Narayanan S (1994) Sialic acid as a tumor marker. Ann Clin Lab Sci 24:376–384Google Scholar
- 9.Wu C, Xia L, Liu L, Qu F, Li G, Sun Z, Ji Z, Sun W, You J (2016) A novel, sensitive and convenient method for determination of sialic acids in human serum utilizing the ultrasonic-assisted closed in-syringe hydrolysis and derivatization prior to high performance liquid chromatography. Anal Methods 8:554–563CrossRefGoogle Scholar
- 18.Warren L (1959) The thiobarbituric acid assay of sialic acids. J Biol Chem 234:1971–1975Google Scholar
- 19.Jourdian GW, Dean L, Roseman S (1971) The sialic acids. XI. A periodate-resorcinol method for the quantitative estimation of free sialic acids and their glycosides. J Biol Chem 246:430–435Google Scholar
- 29.ICH (1996), International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, Q2B Validation of Analytical Procedures: Methodology. ICH-Q2B, 1–10Google Scholar
- 36.AOAC, Guidelines for Standard Method Performance Requirements. AOAC Official Methods of Analysis, AOAC International, Appendix F, 1−17. Gaithersburg, MD, USA, 2016Google Scholar