An organic electrochemical transistor for determination of microRNA21 using gold nanoparticles and a capture DNA probe
A method is described for the determination of microRNA. It is based on the use of organic electrochemical transistors (OECTs) fabricated on a flexible poly(ethylene terephthalate) substrate. A gold electrode was modified with gold nanoparticles to immobilize the capture DNA probe and then served as the gate of the device. The detection of microRNA21 was realized by monitoring the change of the drain-source current after hybridization of capture DNA with microRNA21. Under optimal conditions, this biosensor exhibits good sensitivity and specificity. It works in the 5 pM to 20 nM microRNA concentration range and has a 2 pM detection limit.
KeywordsOrganic bioelectronics Screen-printing Flexible device Nanomaterial surface modification Self-assembling Electrochemical biosensor HeLa cells
This work was supported by the National Natural Science Foundation of China under grants (21275050 and 21145001), the Hunan Provincial Natural Science Foundation of China (13JJ1016), the Scientific Research Fund of Hunan Provincial Education Department (13A053), the Construct Program of the Key Discipline in Hunan Province, and Foundation of the Science & Technology Department of Hunan Province (2016SK2020).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 1.De Pontual L, Yao E, Callier P, Faivre L, Drouin V, Cariou S, Van Haeringen A, Genevieve D, Goldenberg A, Oufadem M, Manouvrier S, Munnich A, Vidigal JA, Vekemans M, Lyonnet S, Henrion-Caude A, Ventura A, Amiel J (2011) Germline deletion of the miR-17~92 cluster causes skeletal and growth defects in humans. Nat Genet 43:1026–1030CrossRefGoogle Scholar
- 12.Liao C, Zhang M, Niu L, Zheng Z, Yan F (2013) Highly selective and sensitive glucose sensors based on organic electrochemical transistors with graphene-modified gate electrodes. J Mater Chem 1:191–200Google Scholar
- 22.Thum T, Gross C, Fielder J, Fischer T, Kissler S, Bussen M, Galuppo P, Just S, Rottbauer W, Frantz S, Castoldi M, Soutschek J, Koteliansky V, Rosenwald A, Basson MA, Licht JD, Pena JTR, Bauersachs J, Engelhardt S (2008) MicroRNA-21 contributes to myocardial disease by stimulating MAP kinase signalling in fibroblasts. Nature 456:980–984CrossRefGoogle Scholar