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Microchimica Acta

, 185:367 | Cite as

Electrochemical dopamine sensor using a nanoporous gold microelectrode: a proof-of-concept study for the detection of dopamine release by scanning electrochemical microscopy

  • Henry Steven Catota Sáenz
  • Lucas Patricio Hernández-Saravia
  • Jéssica S. G. Selva
  • Anandhakumar Sukeri
  • Patricio Javier Espinoza-Montero
  • Mauro Bertotti
Original Paper

Abstract

Nanoporous gold (NPG) structures were prepared on the surface of a gold microelectrode (Au-μE) by an anodization-reduction method. Cyclic voltammetry and field emission scanning electron microscopy were used to study the electrochemical properties and the morphology of the nanostructured film. Voltammetry showed an improved sensitivity for dopamine (DA) oxidation at this microelectrode when compared to a bare gold microelectrode, with a peak near 0.2 V (vs. Ag/AgCl) at a scan rate of 0.1 V s−1. This is due to the increased surface area and roughness. Square wave voltammetry shows a response that is linear in the 0.1–10 μmol L−1 DA concentration range, with a 30 nmol L-1 detection limit and a sensitivity of 1.18 mA (μmol L−1)−1 cm−2. The sensor is not interfered by ascorbic acid. The reproducibility, repeatability, long-term stability and real sample analysis (spiked urine) were assessed, and acceptable performance was achieved. The “proof-of-concept” detection of dopamine release was demonstrated by using scanning electrochemical microscopy (SECM) with the aim of future applications for single cell analysis.

Graphical abstract

A reproducible electrochemical approach was proposed to fabricate an NPG-microelectrode for DA detection, with enhanced sensitivity and selectivity. Besides, a proof-of-concept detection of DA release was also demonstrated by using SECM.

Keywords

Anodization-reduction Large surface area Electrocatalytic activity Square wave voltammetry Cyclic voltammetry Single cell analysis 

Notes

Acknowledgements

The authors thank Sao Paulo Research Foundation (FAPESP), CNPq and CAPES for regular final supports. SAK and MB gratefully acknowledged FAPESP and CNPq for the research grants #2014/15215-5 & #2015/20776-9 and 150177/2018-6, respectively. JSGS thanks to CNPq for a PhD fellowship grant #141866/2016-0. HSCS acknowledges to Oficina de Porgramas y Servicios Internacionales, OPSI, of the Escuela Politécnica Nacional for the funding granted for his stay at Institute of Chemistry, USP-Brazil.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2898_MOESM1_ESM.docx (1 mb)
ESM 1 (DOCX 1.01 mb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Henry Steven Catota Sáenz
    • 1
    • 2
  • Lucas Patricio Hernández-Saravia
    • 1
    • 3
  • Jéssica S. G. Selva
    • 1
  • Anandhakumar Sukeri
    • 1
  • Patricio Javier Espinoza-Montero
    • 4
  • Mauro Bertotti
    • 1
  1. 1.Department of Fundamental Chemistry, Institute of ChemistryUniversity of São PauloSão PauloBrazil
  2. 2.Facultad de Ingeniería Química y AgroindustriaEscuela Politécnica NacionalQuitoEcuador
  3. 3.Department of ChemistryUniversidad de TarapacáAricaChile
  4. 4.Escuela de Ciencias QuímicasPontificia Universidad Católica del EcuadorQuitoEcuador

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