Microchimica Acta

, 185:46 | Cite as

Boronate-modified hollow molecularly imprinted polymers for selective enrichment of glycosides

  • Yue Hu
  • Qinfei Xia
  • Wei Huang
  • Xingyu Hou
  • Miaomiao Tian
Original Paper


D-Glucose was used as a dummy template in a strategy to fabricate boronate-based hollow molecularly imprinted polymers (h-MIPs) for the recognition of glycosides. 3-Aminophenylboronic acid (APBA) was selected as the functional monomer to impart glycoside binding capability. A polystyrene core was synthesized via an emulsifier-free emulsion polymerization. It was then be corroded to form a hollow structure where the binding sites on the inner surface can be fully utilized. Adsorption studies indicate that the resulting h-MIPs can specifically recognize glycosides. The h-MIPs were applied as a sorbent for solid phase extraction of the glycosides (daidzin, glycitin, rutin, and genistin) in soy products. Following desorption with 1% TFA in acetonitrile-water (3:7, v/v), the glycosides were quantified by HPLC with UV detection. The detection limits for the glycosides range from 3.5 to 10.8 ng·mL−1.

Graphical abstract

Boronate-affinity hollow molecularly imprinted polymers using D-glucose as dummy template were prepared as solid phase extraction adsorbents for selective enrichment of glycosides in soy products prior to quantitation by HPLC-UV.


D-glucose Dummy template H-MIPs APBA PS Sorbent Soy products 


Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2017_2608_MOESM1_ESM.doc (4.3 mb)
ESM 1 (DOC 4396 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, Heilongjiang Province, College of Chemistry and Chemical EngineeringHarbin Normal UniversityHarbinPeople’s Republic of China

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