Detection of glutathione based on MnO2 nanosheet-gated mesoporous silica nanoparticles and target induced release of glucose measured with a portable glucose meter
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The authors describe a novel method for the determination of glutathione (GSH). Detection is based on target induced release of glucose from MnO2 nanosheet-gated aminated mesoporous silica nanoparticles (MSNs). In detail, glucose is loaded into the pores of MSNs. Negatively charged MnO2 nanosheets are assembled on the MSNs through electrostatic interactions. The nanosheets are reduced by GSH, and this results in the release of glucose which is quantified by using a commercial electrochemical glucose meter. GSH can be quantified by this method in the 100 nM to 10 μM concentration range, with a 34 nM limit of detection.
KeywordsMnO2 nanosheets Mesoporous silica Personal glucose meter Glutathione Portable detection Target induced release
The authors are grateful for the support of the National Natural Science Foundation of China (21775089, 21375076, 21705151), the Project of Shandong Province Science and Technology Program (2015GSF121031), Outstanding Youth Foundation of Shandong Province (ZR2017JL010), and the Project of Beijing National Science Foundation (2174085).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 1.SC L (1999) Regulation of hepatic glutathione synthesis: current concepts and controversies. FASEB J 13:1169–1183Google Scholar
- 20.Chen S, Zhang J, Gan N, Hu F, Li T, Cao Y, Pan D (2015) An on-site immunosensor for ractopamine based on a personal glucose meter and using magnetic β-cyclodextrin-coated nanoparticles for enrichment, and an invertase-labeled nanogold probe for signal amplification. Microchim Acta 182:815–822CrossRefGoogle Scholar
- 25.Dong Z-Z, Lu L, Ko C-N, Yang C, Li S, Lee M-Y, Leung C-H, Ma D-L (2017) A MnO2 nanosheet-assisted GSH detection platform using an iridium (iii) complex as a switch-on luminescent probe. Nano 9:4677–4682Google Scholar
- 31.Kong X-J, Wu S, Chen T-T, R-Q Y, Chu X (2016) MnO2-induced synthesis of fluorescent polydopamine nanoparticles for reduced glutathione sensing in human whole blood. Nano 8:15604–15610Google Scholar