Amperometric myeloperoxidase immunoassay based on the use of CuPdPt nanowire networks
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This research describes a nanowire network-based method for detecting the activity of myeloperoxidase (MPO), a biomarker of acute coronary syndromes (ACS). Trimetallic CuPdPt nanowire networks (CuPdPt NWNWs) were prepared by a one-step chemical reduction method. The metallic precursors quickly form nanowire network structures without the need for additional capping agents or surfactants. This process creates a product with a clean surface. The NWNWs were dropped onto a glassy carbon electrode (GCE) to obtain a sensor with good catalytic activity towards the reduction of hydrogen peroxide (H2O2), which was used as an electrochemical probe working at −0.4 V (vs. SCE). It also provided a large surface for further modification. Next, an antibody against MPO was immobilized on the modified GCE via the stable conjunction between Cu, Pt, Pd and amino groups. Upon binding of MPO to the antibody on the GCE, the current response to H2O2 was reduced by 35 μA·cm−2. The immunosensor had a linear response within the 100 fg·mL−1 to 50 ng·mL−1 MPO concentration range and a 33 fg·mL−1 detection limit (at an S/N ratio of 3). The recovery of spiked serum samples ranged from 99.8 to 103.6%. This result suggests that the method can be applied to the quantitation of MPO in human serum samples.
KeywordsElectrochemical immunosensor Myeloperoxidase Network-based platform Amperometry One-step method Catalytic activity
We are grateful for the financial support from the National Nature Science Foundation of China (No. 81370403), the Chongqing Foundation and Advanced Research Project (No. CSTC2015jcyjBX0053), the Chongqing Precision Medical Key Technology Research and Development and Demonstration Projects (cstc2016shms-ztzx0042) and the Chongqing Medical University Scientific Research Cultivating Fund (No.201414).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
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