Microchimica Acta

, Volume 184, Issue 7, pp 2181–2189 | Cite as

Photometric determination of free cholesterol via cholesterol oxidase and carbon nanotube supported Prussian blue as a peroxidase mimic

Original Paper
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Abstract

The authors describe a photometric method for the determination of free cholesterol based on the oxidation of cholesterol by the catalytic action of the enzyme cholesterol oxidase. The hydrogen peroxide formed is used to oxidize the chromogenic compound 3,3′,5,5′-tetramethylbenzidine (TMB) by exploiting the peroxidase-like activity of carbon nanotube-supported Prussian blue (PB). This enzyme mimic was synthesized from a mixture of ferric chloride and hexacyanoferrate(III) in the presence of intrinsically reducing multi-walled carbon nanotubes. The composite can trigger the formation of a blue-green dye from TMB in the presence of H2O2. The findings were exploited to design a photometric (652 nm) assay that has a linear response in the 4 to 100 μM cholesterol concentration range and a 3 μM detection limit. The practicability of the method was verified by the successful analysis of free cholesterol in human blood samples.

Graphical abstract

Schematic of a carbon nanotube-supported Prussian blue (PB/MWCNT) hybrid used as a promising peroxidase mimic. It has the advantages of low cost, simple synthesis, high activity, and good stability. PB/MWCNTs along with cholesterol oxidase (ChOx) are used in a colorimetric assay for the determination of free cholesterol.

Keywords

Colorimetric analysis Peroxidase-like activity Nanozyme Hydrogen peroxide Visualized detection Hydroxy radical 
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Notes

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Nos. 21605061 and 31601549), the Natural Science Foundation of Jiangsu Province (No. BK20160489), the Natural Science Fund for Colleges and Universities in Jiangsu Province (No. 16KJB150009), the Postdoctoral Fund of China (No. 2016 M600365), the Postdoctoral Fund of Jiangsu Province (No. 1601015B), the Open Fund from the Shanghai Key Laboratory of Functional Materials Chemistry (No. SKLFMC201601), the Start-Up Research Fund of Jiangsu University (No. 15JDG143), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2017_2235_MOESM1_ESM.docx (1.4 mb)
ESM 1 (DOCX 1477 kb)

References

  1. 1.
    Saric M, Kronzon I (2011) Cholesterol embolization syndrome. Curr Opin Cardiol 26:472–479CrossRefGoogle Scholar
  2. 2.
    Wisitsoraat A, Sritongkham P, Karuwan C, Phokharatkul D, Maturos T, Tuantranont A (2010) Fast cholesterol detection using flow injection microfluidic device with functionalized carbon nanotubes based electrochemical sensor. Biosens Bioelectron 26:1514–1520CrossRefGoogle Scholar
  3. 3.
    Hayat A, Haider W, Raza Y, Marty JL (2015) Colorimetric cholesterol sensor based on peroxidase-like activity of zinc oxide nanoparticles incorporated carbon nanotubes. Talanta 143:157–161CrossRefGoogle Scholar
  4. 4.
    Bui TT, Park SY (2016) A carbon dot-hemoglobin complex-based biosensor for cholesterol detection. Green Chem 18:4245–4253CrossRefGoogle Scholar
  5. 5.
    Tong YJ, Li HD, Guan HM, Zhao JM, Majeed S, Anjum S, Liang F, Xu GB (2013) Electrochemical cholesterol sensor based on carbon nanotube@molecularly imprinted polymer modified ceramic carbon electrode. Biosens Bioelectron 47:553–558CrossRefGoogle Scholar
  6. 6.
    Cai XJ, Gao X, Wang LS, Wu Q, Lin XF (2013) A layer-by-layer assembled and carbon nanotubes/gold nanoparticles-based bienzyme biosensor for cholesterol detection. Sensor Actuat B-Chem 181:575–583CrossRefGoogle Scholar
  7. 7.
    Hanefeld U, Cao LQ, Magner E (2013) Enzyme immobilisation: fundamentals and application. Chem Soc Rev 42:6211–6212CrossRefGoogle Scholar
  8. 8.
    Gao LZ, Zhuang J, Nie L, Zhang JB, Zhang Y, Gu N, Wang TH, Feng J, Yang DL, Perrett S, Yan X (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2:577–583CrossRefGoogle Scholar
  9. 9.
    Yan J, Huang YF, Zhang CH, Fang ZZ, Bai WH, Yan MM, Zhu C, Chen AL (2017) Aptamer based photometric assay for the antibiotic sulfadimethoxine based on the inhibition and reactivation of the peroxidase-like activity of gold nanoparticles. Microchim Acta 184:59–63CrossRefGoogle Scholar
  10. 10.
    Pan N, Li-Ying W, Wu LL, Peng CF, Xie ZJ (2017) Colorimetric determination of cysteine by exploiting its inhibitory action on the peroxidase-like activity of au@Pt core-shell nanohybrids. Microchim Acta 184:65–72CrossRefGoogle Scholar
  11. 11.
    Niu XH, He YF, Pan JM, Li X, Qiu FX, Yan YS, Shi LB, Zhao HL, Lan MB (2016) Uncapped nanobranch-based CuS clews used as an efficient peroxidase mimic enable the visual detection of hydrogen peroxide and glucose with fast response. Anal Chim Acta 947:42–49CrossRefGoogle Scholar
  12. 12.
    Ding CP, Yan YH, Xiang DS, Zhang CL, Xian YZ (2016) Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay. Microchim Acta 183:625–631CrossRefGoogle Scholar
  13. 13.
    Espinosa JC, Navalon S, Primo A, Moral M, Sanz JF, Alvaro M, Garcia H (2015) Graphenes as efficient metal-free fenton catalysts. Chem Eur J 21:11966–11971CrossRefGoogle Scholar
  14. 14.
    Chen J, Chen Q, Chen JY, Qiu HD (2016) Magnetic carbon nitride nanocomposites as enhanced peroxidase mimetics for use in colorimetric bioassays, and their application to the determination of H2O2 and glucose. Microchim Acta 183:3191–3199CrossRefGoogle Scholar
  15. 15.
    Wei H, Wang EK (2013) Nanomaterials with enzyme-like characteristics (nanoenzymes): next-generation artificial enzymes. Chem Soc Rev 42:6060–6093CrossRefGoogle Scholar
  16. 16.
    Nasir M, Nawaz MH, Latif U, Yaqub M, Hayat A, Rahim A (2017) An overview on enzyme-mimicking nanomaterials for use in electrochemical and optical assays. Microchim Acta 184:323–342CrossRefGoogle Scholar
  17. 17.
    Zhao Y, Qiang H, Chen ZB (2017) Colorimetric determination of hg(II) based on a visually detectable signal amplification induced by a cu@au-hg trimetallic amalgam with peroxidase-like activity. Microchim Acta 184:107–115CrossRefGoogle Scholar
  18. 18.
    Ferlay S, Mallah T, Ouahes R, Veillet P, Verdaguer M (1995) A room-temperature organometallic magnet based on Prussian blue. Nature 378:701–703CrossRefGoogle Scholar
  19. 19.
    DeLongchamp DM, Hammond PT (2004) High-contrast electrochromism and controllable dissolution of assembled Prussian blue/polymer nanocomposites. Adv Funct Mater 14:224–232CrossRefGoogle Scholar
  20. 20.
    Kong B, Selomulya C, Zheng GF, Zhao DY (2015) New faces of porous Prussian blue: interfacial assembly of integrated hetero-structures for sensing applications. Chem Soc Rev 44:7997–8018CrossRefGoogle Scholar
  21. 21.
    Čunderlová V, Hlaváček A, Horňáková V, Peterek M, Němeček D, Hampl A, Eyer L, Skládal P (2015) Catalytic nanocrystalline coordination polymers as an efficient peroxidase mimic for labeling and optical immunoassays. Microchim Acta 183:651–658CrossRefGoogle Scholar
  22. 22.
    Karyakin AA, Karyakina EE (1999) Prussian blue-based ‘artificial peroxidase’ as a transducer for hydrogen peroxide detection: application to biosensors. Sensor. Actuat. B-Chem. 57:268–273CrossRefGoogle Scholar
  23. 23.
    Liu Y, Chu ZY, Jin WQ (2009) A sensitivity-controlled hydrogen peroxide sensor based on self-assembled Prussian blue modified electrode. Electrochem Commun 11:484–487CrossRefGoogle Scholar
  24. 24.
    Wang T, Fu YC, Chai LY, Chao L, Bu LJ, Meng Y, Chen C, Ma M, Xie QJ, Yao SZ (2014) Filling carbon nanotubes with Prussian blue nanoparticles of high peroxidase-like catalytic activity for colorimetric chemoand biosensing. Chem Eur J 20:2623–2630CrossRefGoogle Scholar
  25. 25.
    Dujardin E, Ebbesen TW, Krishnan A, Treacy MMJ (1998) Purification of single-shell nanotubes. Adv Mater 10:611–613CrossRefGoogle Scholar
  26. 26.
    Taguchi M, Yagi I, Nakagawa M, Iyoda T, Einaga Y (2006) Photocontrolled magnetization of CdS-modified Prussian blue nanoparticles. J Am Chem Soc 128:10978–10982CrossRefGoogle Scholar
  27. 27.
    Nie P, Shen LF, Luo HF, Ding B, Xu GY, Wang J, Zhang XG (2014) Prussian blue analogues: a new class of anode materials for lithium ion batteries. J Mater Chem A 2:5852–5857CrossRefGoogle Scholar
  28. 28.
    Zhang WM, Ma D, Du JX (2014) Prussian blue nanoparticles as peroxidase mimetics for sensitive colorimetric detection of hydrogen peroxide and glucose. Talanta 120:362–367CrossRefGoogle Scholar
  29. 29.
    Ding N, Yan N, Ren CL, Chen XG (2010) Colorimetric determination of melamine in dairy products by Fe3O4 magnetic nanoparticles-H2O2-ABTS detection system. Anal Chem 82:5897–5899CrossRefGoogle Scholar
  30. 30.
    Cheng HJ, Zhang L, He J, Guo WJ, Zhou ZY, Zhang XJ, Nie SM, H. Wei (2016) Integrated nanozymes with nanoscale proximity for in vivo neurochemical monitoring in living brains. Anal Chem 88:5489–5497Google Scholar
  31. 31.
    Shi WJ, Fan H, Ai SY, Zhu LS (2015) Honeycomb-like nitrogen-doped porous carbon supporting Pt nanoparticles as enzyme mimic for colorimetric detection of cholesterol. Sensor. Actuat. B-Chem. 221:1515–1522CrossRefGoogle Scholar
  32. 32.
    Nirala NR, Pandey S, Bansal A, Singh VK, Mukherjee B, Saxena PS, Srivastava A (2015) Different shades of cholesterol: gold nanoparticles supported on MoS2 nanoribbons for enhanced colorimetric sensing of free cholesterol. Biosens Bioelectron 74:207–213CrossRefGoogle Scholar
  33. 33.
    Nirala NR, Abraham S, Kumar V, Bansal A, Srivastava A, Saxena PS (2015) Colorimetric detection of cholesterol based on highly efficient peroxidase mimetic activity of graphene quantum dots. Sensor. Actuat. B-Chem. 218:42–50CrossRefGoogle Scholar
  34. 34.
    Tyagi M, Chandran A, Joshi T, Prakash J, Agrawal VV, Biradar AM (2014) Self assembled monolayer based liquid crystal biosensor for free cholesterol detection. Appl Phys Lett 104:154104CrossRefGoogle Scholar
  35. 35.
    Lee YJ, Park JY (2010) Nonenzymatic free-cholesterol detection via a modified highly sensitive macroporous gold electrode with platinum nanoparticles. Biosens Bioelectron 26:1353–1358CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  1. 1.Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical EngineeringJiangsu UniversityZhenjiangChina
  2. 2.Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular EngineeringEast China University of Science and TechnologyShanghaiChina
  3. 3.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiChina

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