Microchimica Acta

, Volume 184, Issue 7, pp 2063–2071 | Cite as

Chelation-enhanced fluorescence of phosphorus doped carbon nanodots for multi-ion detection

Original Paper

Abstract

The present paper reports on a chelation enhanced fluorescence (CHEF) effect that is observed on addition of certain metal ions to phosphorus doped carbon nanodots (P-CNDs). The effect is accompanied by a large shortwave shift of the emission peak. Highly passivated P-CNDs with sizes of around 3 nm were prepared from lactose and phosphoric acid, using a one-pot low temperature solvothermal method. The nanoparticles were purified according to polarity and size. The extent of blue shift and strength of enhancement depend on metal ions and actual pH value. For instance, the P-CND complex with Al(III) has a fluorescence that is shifted to shorter wavelengths, and the fluorescence quantum yield is enhanced from 12% (for the free P-CNDs) to almost 62% at 490 nm. The fluorescence is also enhanced and shifted by the ions Zn(II) and Cd(II). It is quenched by the ions Fe(II), Fe(III), Hg(II), Cu(II) and Sn(II), among others. The enhancement is attributed to the chelation of metal ions with the passivated surface functional groups of P-CNDs, mainly those of phosphorus. Phosphorous free CNDs (prepared via HCl instead of H3PO4) and low-passivated P-CNDs (prepared for longer period of time; typically 8 h) show no enhancement. The metal ion induced enhancement led to the design of a fluorometric assay for the detection of these ions. The detection limits are 4 nM for Al(III) and 100 nM for Zn(II). The two ions were quantified in spiked pharmaceutical formulations. Recoveries typically are 102% (for n = 7).

Graphical abstract

The fluorescence emission of phosphorous doped carbon nanodots is significantly enhanced and tuned after binding to Al3+, Zn2+ and Cd2+. The enhancement mechanism is attributed to chelation enhanced fluorescence (CHEF).

Keywords

Carbon nanodots Enhancement CHEF Fluorescence enhancement Phosphorus doped carbon dots Quantum yield 

Supplementary material

604_2017_2196_MOESM1_ESM.docx (7 mb)
ESM 1(DOCX 6.97 mb)

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Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  1. 1.Department of Chemistry, College of ScienceUniversity of SulaimaniSulaymaniaIraq
  2. 2.Komar Research CenterKomar University of Science and TechnologySulaymania CityIraq

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