Microchimica Acta

, Volume 184, Issue 2, pp 595–601 | Cite as

SERS assay for pyrophosphate based on its competitive binding to Cu(II) ion on silver nanoparticles modified with cysteine and rhodamine 6G

  • Lan Luo
  • Yanhua Chen
  • Lixia Zhang
  • Yaru Li
  • Hongli Li
  • Hanqi Zhang
  • Yuan Tian
Original Paper
First Online:
Received:
Accepted:

Abstract

The authors have developed a surface-enhanced Raman spectroscopic (SERS) method for the determination of pyrophosphate ion (PPi). It is based on the competitive coordination of Cu(II) between cysteine (Cys) and PPi using silver nanoparticles (AgNPs) modified with Cys and Rhodamine 6G (R6G). Cys was attached to the surface of the AgNPs via Ag−S bond, and the amino acid unit in Cys bind to Cu(II) to form a chelate complex. This results in the aggregation of AgNPs and a strong SERS signal for the probe R6G. However, in the presence of PPi, the aggregated AgNPs are solubilized because of the stronger affinity between PPi and Cu(II). This leads to a decrease of the SERS signal and forms the basis for the quantitation of PPi. The amount of AgNPs, the concentration of Cu(II), and the mixing time were optimized. The method displays a linear response in the 0.1 to 80 μM PPi concentration range, and the limit of detection is as low as 20 nM. The method was applied to the determination of PPi in spiked serum samples and urine samples, with recoveries between 95.2 to 100.5% and relative standard deviations of <4.4%.

Graphical abstract

The cysteine and R6G functionalized silver nanoparticles (AgNPs) were synthesized as a surface-enhanced Raman spectroscopic (SERS) substrate to detect pyrophosphate ion (PPi). It is based on the competitive coordination of Cu(II) between cysteine (Cys) and PPi.

Keywords

Surface-enhanced Raman scattering SERS Surface functionalization Particle aggregation Particle deaggregation Urine analysis Serum analysis 
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Notes

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2016_2044_MOESM1_ESM.doc (15.3 mb)
ESM 1 (DOC 15.2 mb)

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Lan Luo
    • 1
  • Yanhua Chen
    • 1
  • Lixia Zhang
    • 1
  • Yaru Li
    • 1
  • Hongli Li
    • 1
  • Hanqi Zhang
    • 1
  • Yuan Tian
    • 1
  1. 1.College of ChemistryJilin UniversityChangchunPeople’s Republic of China

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