Electrochemical aptamer based assay for the neonicotinoid insecticide acetamiprid based on the use of an unmodified gold electrode
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The authors report on an aptamer-based electrochemical assay for the insecticide acetamiprid. It is based on the target-induced release of the redox probe Methylene Blue (MB) from the dsDNA formed between aptamer and complementary strand (Apt/CS), exonuclease I (Exo I) and silica nanoparticles coated with streptavidin (SiNP-Streptavidin). MB is detected electrochemically using an unmodified gold electrode. In the presence of acetamiprid, MB is released from the Apt/CS dsDNA and accumulated in the close environment of the gold electrode. This results in a strong electrochemical signal for MB at fairly low working voltage of typically −0.27 V. In the absence of target, however, the SiNP-streptavidin conjugate modified MB-dsDNA remains intact. Hence, the electrochemical signal remains weak. The method displays high selectivity for acetamiprid and a limit of detection as low as 153 pM. The assay was successfully applied to the determination of acetamiprid in (spiked) water and serum samples, with LODs of 161 and 209 pM, respectively.
KeywordsMethylene blue Differential pulse voltammetry Silica nanoparticles Insecticide Exonuclease I Complementary strand Water analysis Serum analysis
Financial support of this study was provided by Mashhad University of Medical Sciences.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 6.Fei A, Liu Q, Huan J, Qian J, Dong X, Qiu B, Mao H, Wang K (2015) Label-free impedimetric aptasensor for detection of femtomole level acetamiprid using gold nanoparticles decorated multiwalled carbon nanotube-reduced graphene oxide nanoribbon composites. Biosens Bioelectron 70:122–129. doi: 10.1016/j.bios.2015.03.028 CrossRefGoogle Scholar
- 8.Lin X, Leung KH, Lin L, Lin S, Leung CH, Ma DL, Lin JM (2016) Determination of cell metabolite VEGF165 and dynamic analysis of protein-DNA interactions by combination of microfluidic technique and luminescent switch-on probe. Biosens Bioelectron 79:41–47. doi: 10.1016/j.bios.2015.11.089 CrossRefGoogle Scholar
- 9.Huo Y, Qi L, Lv XJ, Lai T, Zhang J, Zhang ZQ (2016) A sensitive aptasensor for colorimetric detection of adenosine triphosphate based on the protective effect of ATP-aptamer complexes on unmodified gold nanoparticles. Biosens Bioelectron 78:315–320. doi: 10.1016/j.bios.2015.11.043 CrossRefGoogle Scholar
- 14.Mohammad Danesh N, Ramezani M, Sarreshtehdar Emrani A, Abnous K, Taghdisi SM (2016) A novel electrochemical aptasensor based on arch-shape structure of aptamer-complimentary strand conjugate and exonuclease I for sensitive detection of streptomycin. Biosens Bioelectron 75:123–128. doi: 10.1016/j.bios.2015.08.017 CrossRefGoogle Scholar
- 15.Taghdisi SM, Danesh NM, Emrani AS, Ramezani M, Abnous K (2015) A novel electrochemical aptasensor based on single-walled carbon nanotubes, gold electrode and complimentary strand of aptamer for ultrasensitive detection of cocaine. Biosens Bioelectron 73:245–250. doi: 10.1016/j.bios.2015.05.065 CrossRefGoogle Scholar
- 16.Emrani AS, Danesh NM, Lavaee P, Ramezani M, Abnous K, Taghdisi SM (2016) Colorimetric and fluorescence quenching aptasensors for detection of streptomycin in blood serum and milk based on double-stranded DNA and gold nanoparticles. Food Chem 190:115–121. doi: 10.1016/j.foodchem.2015.05.079 CrossRefGoogle Scholar
- 18.Emrani AS, Danesh NM, Ramezani M, Taghdisi SM, Abnous K (2016) A novel fluorescent aptasensor based on hairpin structure of complementary strand of aptamer and nanoparticles as a signal amplification approach for ultrasensitive detection of cocaine. Biosens Bioelectron 79:288–293. doi: 10.1016/j.bios.2015.12.025 CrossRefGoogle Scholar
- 27.Yan Z, Gan N, Li T, Cao Y, Chen Y (2016) A sensitive electrochemical aptasensor for multiplex antibiotics detection based on high-capacity magnetic hollow porous nanotracers coupling exonuclease-assisted cascade target recycling. Biosens Bioelectron 78:51–57. doi: 10.1016/j.bios.2015.11.019 CrossRefGoogle Scholar