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Microchimica Acta

, Volume 184, Issue 1, pp 203–210 | Cite as

Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots

  • Yashan Wang
  • Tiancong Ma
  • Shuyue Ma
  • Yongjun Liu
  • Yaping Tian
  • Ruinan Wang
  • Yanbin Jiang
  • Dongjun HouEmail author
  • Jianlong WangEmail author
Original Paper

Abstract

A rapid and sensitive aptamer-based assay is described for kanamycin, a veterinary antibiotic with neurotoxic side effects. It is based on a novel FRET pair consisting of fluorescent carbon dots and layered MoS2. This donor-acceptor pair (operated at excitation/emission wavelengths of 380/440 nm) shows fluorescence recovery efficiencies reaching 93 %. By taking advantages of aptamer-induced fluorescence quenching and recovery, kanamycin can be quantified in the of 4–25 μM concentration range, with a detection limit of 1.1 μM. The method displays good specificity and was applied to the determination of kanamycin in spiked milk where it gave recoveries ranging from 85 % to 102 %, demonstrating that the method serves as a promising tool for the rapid detection of kanamycin in milk and other animal-derived foodstuff.

Graphical Abstract

A fluorometric aptasensor was developed for the determination of kanamycin. It is based on a novel FRET pair of carbon dots and layered MoS2. The fluorescence recovery efficiency reached 93 % with a good sensitivity, specificity and recoveries in spiked milk.

Keywords

Veterinary antibiotic Aptasensor Molybdenum disulfide MoS2 Exfoliation Fluorescence resonance energy transfer (FRET) Milk analysis 

Notes

Acknowledgments

This work is supported by the National Science & Technology Pillar Program of China (No. 2014BAD13B05) and the Yangling Agricultural Hi-tech Industries Demonstration Zone (2014NY-35).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Yashan Wang
    • 1
  • Tiancong Ma
    • 2
  • Shuyue Ma
    • 3
  • Yongjun Liu
    • 1
  • Yaping Tian
    • 1
  • Ruinan Wang
    • 1
  • Yanbin Jiang
    • 1
  • Dongjun Hou
    • 1
    Email author
  • Jianlong Wang
    • 3
    Email author
  1. 1.Ministry of AgricultureChina Animal Disease Control CenterBeijingChina
  2. 2.Institute of ChemistryChinese Academy of SciencesBeijingChina
  3. 3.College of Food Science and EngineeringNorthwest A&F UniversityYanglingChina

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