Microchimica Acta

, Volume 183, Issue 9, pp 2501–2508 | Cite as

Temperature-responsive amperometric H2O2 biosensor using a composite film consisting of poly(N-isopropylacrylamide)-b-poly (2-acrylamidoethyl benzoate), graphene oxide and hemoglobin

  • Yuanqing Zhou
  • Jing Cao
  • Jia Zhao
  • Yixi Xie
  • Junjie FeiEmail author
  • Yuanli Cai
Original Paper


A temperature-responsive biosensing film consisting of the temperature-responsive block co-polymer poly (N-isopropylacrylamide)-b-poly(2-acrylamidoethyl benzoate) (referred to as PNIPAM-b-PAAE), graphene oxide (GO), and hemoglobin (Hb) was fabricated and used to modify a glassy carbon electrode (GCE). The film provides a favorable micro-environment for Hb to facilitate the electron transfer to the GCE. Hb at PNIPAM-b-PAAE/GO/Hb (PGH) film exhibits a couple of well-defined redox peaks with a formal potential of −0.371 V (vs. SCE) and displays intrinsic electro-catalytic activity toward H2O2. The sensing film also shows temperature-tunable catalytic activity toward H2O2 that can be stimulated by temperature. Large peak currents can be seen in amperometry at 0.4 V (vs. SCE) in pH 7.0 phosphate buffer only if the temperature is above the lower critical solution temperature (LCST) of 32 °C. The response of the modified GCE is linear in the 0.1 to 3.7 μmol L−1 concentration range if operated at above 32 °C, but in the 0.2 to 3.7 μmol L−1 concentration range at below 30 °C. This behavior is attributed to the temperature-dependent phase transition of PNIPAM-b-PAAE and cooperative effect of GO. The strategy presented here in our perception meets the requirements of switchable sensors for use in bioscience and biotechnology.

Graphical abstract

A temperature-responsive biosensing film consisting of temperature-responsive polymer, graphene oxide and hemoglobin has been fabricated. This film displays favorable electrochemical property and good electro-catalytic activity toward H2O2. It also exhibits catalytic activity change upon temperature stimuli.


Temperature-responsive polymer On-off detection Electrochemical switch Glassy carbon electrode Cyclic voltammetry 



This research was financially supported by the NSF of China (Grants No. 21475114 and 21275123), Program for Changjiang Scholars and Innovative Research Team in University (1337304), Project of Hunan provincial natural science Foundation of China (14JJ1019), Research fund for the doctoral program of higher education of china, Ministry of education of China (20134301110005).

Supplementary material

604_2016_1893_MOESM1_ESM.doc (803 kb)
ESM 1 (DOC 803 kb)


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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Yuanqing Zhou
    • 1
  • Jing Cao
    • 1
  • Jia Zhao
    • 2
  • Yixi Xie
    • 1
  • Junjie Fei
    • 1
    Email author
  • Yuanli Cai
    • 1
    • 3
  1. 1.Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of ChemistryXiangtan UniversityXiangtanPeople’s Republic of China
  2. 2.College of Resource and EnvironmentHunan Agricultural UniversityChangshaPeople’s Republic of China
  3. 3.Department of Polymer Science and EngineeringSoochow UniversitySuzhouPeople’s Republic of China

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