A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles
- 637 Downloads
The authors describe an aptasensor for the detection of the gonadal hormone progesterone (P4) in aqueous solution. Gold nanoparticles (AuNPs) were coated with a P4-specific aptamer, and such particles do not aggregate in presence of NaCl due to the presence of the aptamer coating. If, however, progesterone is added, it will bind to the aptamer and release it from the surface. The uncoated AuNPs, on addition of NaCl, will aggregate and a color change from red (520 nm) to blue (650 nm) can be visually detected or photometrically quantified. The ratio of the absorbances at 650 and 520 nm is linearly related to the P4 concentration in the range from 2.6 to 800 nM. The complete detection range extends from 2.6 to 1400 nM, and the detection limit is 2.6 nM. Water containing various potential interferents, as well as tap water and urine, were spiked with P4 and the recoveries of P4 are in the range of 89.7–117.5%, 84.4–115.0% and 94.7–118.8%, respectively. This assay has a large potential with respect to the visual and instrumental determination of P4 in aquatic environment and urine.
KeywordsPregnancy marker Aptamer Assay AuNPs Aggregation Rapid testing Visual test
This work was sponsored by the Natural Science Foundation of Shanghai (13ZR1421700), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, and National Natural Science Foundation of China (20977062 & 31201682).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 6.Ren S, Wang X, Lin Z, Li Z, Ying X, Chen G, Lin JM (2008) Development of a high-throughput, indirect antibody immobilization format chemiluminescence enzyme immunoassay (CLEIA) for the determination of progesterone in human serum. Lumin J Biol Chem Lumin 23(3):175–181. doi: 10.1002/bio.1031 CrossRefGoogle Scholar
- 7.Sun L, Yong W, Chu X, Lin JM (2009) Simultaneous determination of 15 steroidal oral contraceptives in water using solid-phase disk extraction followed by high performance liquid chromatography-tandem mass spectrometry. J Chromatogr A 1216(28):5416–5423. doi: 10.1016/j.chroma.2009.05.041 CrossRefGoogle Scholar
- 14.Freeman R, Liu X, Willner I (2011) Chemiluminescent and chemiluminescence resonance energy transfer (CRET) detection of DNA, metal ions, and aptamer-substrate complexes using hemin/G-quadruplexes and CdSe/ZnS quantum dots. J Am Chem Soc 133(30):11597–11604. doi: 10.1021/ja202639m CrossRefGoogle Scholar
- 15.Zhan X, Hu G, Wagberg T, Zhan S, Xu H, Zhou P (2016) Electrochemical aptasensor for tetracycline using a screen-printed carbon electrode modified with an alginate film containing reduced graphene oxide and magnetite (Fe3O4) nanoparticles. Microchim Acta 183(2). doi: 10.1007/s00604-015-1718-y
- 24.Zhan S, Wu Y, He L, Wang F, Zhan X, Zhou P, Qiu S (2012) A silver-specific DNA-based bio-assay for Ag(Ι) detection via the aggregation of unmodified gold nanoparticles in aqueous solution coupled with resonance Rayleigh scattering. Anal Methods 4(12):3997–4002. doi: 10.1039/c2ay25403d CrossRefGoogle Scholar
- 33.Jin R, Wu W, Li Z, Mirkin CA, Schatz GC, What controls the melting properties of DNA-linked goldnanoparticles assemblies? Journal of the American Chemical Society 125 (6):1643–1654. doi: 10.1021/ja021096v
- 34.Duy SV, Fayad PB, Barbeau B, Prévost M, Sauvé S (2012) Using a novel sol-gel stir bar sorptive extraction method for the analysis of steroid hormones in water by laser diode thermal desorption/atmospheric chemical ionization tandem mass spectrometry. Talanta 101:337–345. doi: 10.1016/j.talanta.2012.09.036 CrossRefGoogle Scholar