A glassy carbon electrode modified with a composite consisting of reduced graphene oxide, zinc oxide and silver nanoparticles in a chitosan matrix for studying the direct electron transfer of glucose oxidase and for enzymatic sensing of glucose
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The authors describe the fabrication of a nanocomposite consisting of reduced graphene oxide, zinc oxide and silver nanoparticles by microwave-assisted synthesis. The composite was further reduced in-situ with hydrazine hydrate and then placed, along with the enzyme glucose oxidase, on a glassy carbon electrode. The synergistic effect of the materials employed in the nanocomposite result in excellent electrocatalytic activity. The Michaelis-Menten constant of the adsorbed GOx is 0.25 mM, implying a remarkable affinity of the GOx for glucose. The amperometric response of the modified GCE is linearly proportional to the concentration of glucose in 0.1 to 12.0 mM concentration range, and the detection limit is 10.6 µM. The biosensor is highly selective, well reproducible and stable.
KeywordsBiosensor Bioassay Enzymatic assay Nanocomposite Microwave synthesis In-situ reduction Hydrazine reduction Direct electrochemistry Amperometry
The work reported here was supported by the National Natural Science Foundation of China under Grant No.51572137, 51502149, 51272117, 51172115, the Natural Science Foundation of Shandong Province under Grant No.ZR2015PE003, ZR2013EMQ006, the Research Award Fund for Outstanding Young Scientists of Shandong Province Grant No. BS2013CL040, the Specialized Research Fund for the Doctoral Program of Higher Education of China under Grant No. 20123719110003, the Tackling Key Program of Science and Technology in Shandong Province under Grant No. 2012GGX1021, the Application Foundation Research Program of Qingdao under Grant No. 13-1-4-117-jch, 15-9-1-28-jch, 14-2-4-29-jch, Shandong Province Taishan Scholar Project. We express our grateful thanks to them for their financial support.
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The author(s) declare that they have no competing interests
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