Microchimica Acta

, Volume 183, Issue 4, pp 1417–1423 | Cite as

Magnetic restricted-access microspheres for extraction of adrenaline, dopamine and noradrenaline from biological samples

  • Deli Xiao
  • Shubo Liu
  • Liyun Liang
  • Yanping BiEmail author
Original Paper


Epoxy propyl bonded magnetic microspheres were prepared by atomic layer deposition using Fe3O4@SiO2 microspheres as a core support material. Then, a restricted-access magnetic sorbent was prepared that contains diol groups on the external surface and m-aminophenylboronic acid groups on the internal surface. This kind of microspheres achieved excellent specific adsorption of the ortho-dihydroxy compounds (dopamine, adrenaline and noradrenaline). Following desorption with sorbitol, the ortho-dihydroxy compounds were quantified by HPLC. The limits of detection for dopamine, adrenaline and noradrenaline were 0.074, 0.053 and 0.095 μg mL−1, respectively. Recoveries from spiked mice serum samples range from 80.2 to 89.1 %.

Graphical Abstract

A magnetic restricted-access sorbent with diol groups on the external surface and m-aminophenylboronic acid groups on the internal surface was synthesized for highly selective extraction of adrenaline, dopamine and noradrenaline from biological samples.


Restricted-access materials Magnetic microspheres Boronic acid Solid-phase extraction Core-shell microspheres Fe3O4@SiO2 Scanning electron microscopy 



This work was supported by the National Natural Science Foundation of China (Grant No. 81402899) and Shandong Provincial Natural Science Foundation, China (No. ZR2014HP020).


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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Deli Xiao
    • 1
    • 2
  • Shubo Liu
    • 1
  • Liyun Liang
    • 1
  • Yanping Bi
    • 3
    Email author
  1. 1.Department of Analytical ChemistryChina Pharmaceutical UniversityNanjingChina
  2. 2.Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of EducationChina Pharmaceutical UniversityNanjingChina
  3. 3.School of Pharmaceutical SciencesTaishan Medical UniversityTai’anChina

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