Magnetic restricted-access microspheres for extraction of adrenaline, dopamine and noradrenaline from biological samples
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Epoxy propyl bonded magnetic microspheres were prepared by atomic layer deposition using Fe3O4@SiO2 microspheres as a core support material. Then, a restricted-access magnetic sorbent was prepared that contains diol groups on the external surface and m-aminophenylboronic acid groups on the internal surface. This kind of microspheres achieved excellent specific adsorption of the ortho-dihydroxy compounds (dopamine, adrenaline and noradrenaline). Following desorption with sorbitol, the ortho-dihydroxy compounds were quantified by HPLC. The limits of detection for dopamine, adrenaline and noradrenaline were 0.074, 0.053 and 0.095 μg mL−1, respectively. Recoveries from spiked mice serum samples range from 80.2 to 89.1 %.
KeywordsRestricted-access materials Magnetic microspheres Boronic acid Solid-phase extraction Core-shell microspheres Fe3O4@SiO2 Scanning electron microscopy
This work was supported by the National Natural Science Foundation of China (Grant No. 81402899) and Shandong Provincial Natural Science Foundation, China (No. ZR2014HP020).
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