Electrochemical aptasensor for tetracycline using a screen-printed carbon electrode modified with an alginate film containing reduced graphene oxide and magnetite (Fe3O4) nanoparticles
- 791 Downloads
The authors describe a label-free electrochemical aptasensor for tetracycline (TET). The TET-binding aptamer was immobilized on a composite consisting of reduced graphene oxide, magnetite (Fe3O4) and sodium alginate, and this material was used to modify the surface of a screen-printed carbon electrode (SPCE). Cyclic voltammetry was carried out to characterize the single steps in the preparation of the modified electrode and to optimize the conditions for detection. Differential pulse voltammetry (DPV) was then used to monitor the interaction between aptamer and TET by applying the electrochemical probe thionine. Under optimal conditions, TET can be quantified by DPV in the 1 nM to 5 μM concentration range, with a detection limit as low as 0.6 nM (at an S/N ratio of 3). The method is rapid, cost-efficient, highly sensitive and specific, and therefore is considered to be a viable platform for TET analysis in food, environmental, and clinical samples.
KeywordsCyclic voltammetry Differential pulse voltammetry Scanning electron microscopy Transmission electron microscopy Sodium alginate Thionine Redox probe
This work was supported by the National High Technology Research and Development Program (“863” Program) of China (No. 2012AA101405), Morningstar Fund of Shanghai Jiaotong University (No. 13X100010018), 1000-Talent Program (Recruitment Program of Global Expert, In Chinese: Qian-Ren-Ji-Hua) and Director Foundation of XTIPC, CAS (No. 2015RC012). We thank Kecheng Cao of Sichuan University for providing the rGO-Fe3O4 and helpful discussions. We also thank Bin Chen of Shanghai Jiaotong University for TEM, HRTEM and EDS analyses.
- 10.Yudthavorasit S, Chiaochan C, Leepipatpiboon N (2011) Simultaneous determination of multi-class antibiotic residues in water using carrier-mediated hollow-fiber liquid-phase microextraction coupled with ultra-high performance liquid chromatography tandem mass spectrometry. Microchim Acta 172(1–2):39–49CrossRefGoogle Scholar
- 14.Velasco-Garcia M, Missailidis S (2009) New trends in aptamer-based electrochemical biosensors. Gene Ther Mol Biol 13:1–10Google Scholar
- 15.Xue F, Wu J, Chu H, Mei Z, Ye Y, Liu J, Zhang R, Peng C, Zheng L, Chen W (2012) Electrochemical aptasensor for the determination of bisphenol a in drinking water. Microchim Acta 180(1–2):109–115Google Scholar
- 18.Zhang H, Shuang S, Sun L, Chen A, Qin Y, Dong C (2013) Label-free aptasensor for thrombin using a glassy carbon electrode modified with a graphene-porphyrin composite. Microchim Acta 181(1–2):189–196Google Scholar
- 28.Cao KC (2015) Preparation of graphene/graphene based hybrid material by microwave assisted methods and the control of defect in graphene. Sichuan University, Chengdu, China, Master thesisGoogle Scholar
- 35.Guo Y, Wang X, Sun X (2015) A label-free electrochemical aptasensor based on electrodeposited gold nanoparticles and methylene blue for tetracycline detection. Int J Electrochem Sci 10:3668–3679Google Scholar