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Microchimica Acta

, Volume 183, Issue 2, pp 625–631 | Cite as

Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay

  • Caiping Ding
  • Yinghan Yan
  • Dongshan Xiang
  • Cuiling ZhangEmail author
  • Yuezhong XianEmail author
Original Paper

Abstract

Greigite magnetic nanoparticles (Fe3S4-MNPs) were prepared and reveal a peroxidase-like activity. Kinetic studies revealed a pseudo-enzymatic activity that is much higher than that of other magnetic nanomaterial-based enzyme mimetics. This finding was exploited to design a photometric enzymatic glucose assay based on the formation of H2O2 during enzymatic oxidation of glucose by glucose oxidase, and the formation of a blue product from an enzyme substrate that is catalytically oxidized by H2O2 in the presence of Fe3S4-MNPs. Glucose can be detected in the 2 to 100 μM concentration range, and the low detection limit is 0.16 μM. The method was applied to quantify glucose in human serum. In our perception, this enzyme mimetic has a large potential in that it may be used in other oxidase based assays, but also in ELISAs.

Graphic Abstract

Fe3S4 magnetic nanoparticles (MNPs) are shown to act as peroxidase mimetics and this was used to design a glucose oxidase (GOx) based glucose assay where the H2O2 formed during oxidation of glucose oxidizes tetramethylbenzidine (TMB) to give a blue product which can be quantified by photometry.

Keywords

Greigite nanoparticles Peroxidase mimic Hydrogen peroxide Glucose assay SEM TEM 

Notes

Acknowledgments

We acknowledge financial support from the National Natural Science Foundation of China (21175046, 21465010) and the Shanghai Natural Science Foundation (15ZR1411600).

Supplementary material

604_2015_1690_MOESM1_ESM.docx (626 kb)
ESM 1 (DOCX 625 kb)

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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  1. 1.Department of Chemistry, School of Chemistry and Molecular EngineeringEast China Normal UniversityShanghaiChina
  2. 2.School of Chemical and Environmental EngineeringHubei University for NationalitiesEnshiChina

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