Signal enhancement in amperometric peroxide detection by using graphene materials with low number of defects
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Two-dimensional carbon nanomaterials ranging from single-layer graphene to defective structures such as chemically reduced graphene oxide were studied with respect to their use in electrodes and sensors. Their electrochemical properties and utility in terms of fabrication of sensing devices are compared. Specifically, the electrodes have been applied to reductive amperometric determination of hydrogen peroxide. Low-defect graphene (SG) was obtained through mechanical exfoliation of natural graphite, while higher-defect graphenes were produced by chemical vapor deposition (CVDG) and by chemical oxidation of graphite and subsequent reduction (rGO). The carbonaceous materials were mainly characterized by Raman microscopy. They were applied as electrode material and the electrochemical behavior was investigated by chronocoulometry, cyclic voltammetry, electrochemical impedance spectroscopy and amperometry and compared to a carbon disc electrode. It is shown that the quality of the graphene has an enormous impact on the amperometric performance. The use of carbon materials with many defects (like rGO) does not result in a significant improvement in signal compared to a plain carbon disc electrode. The sensitivity is 173 mA · M−1 · cm−2 in case of using CVDG which is about 50 times better than that of a plain carbon disc electrode and about 7 times better than that of rGO. The limit of detection for hydrogen peroxide is 15.1 μM (at a working potential of −0.3 V vs SCE) for CVDG. It is concluded that the application of two-dimensional carbon nanomaterials offers large perspectives in amperometric detection systems due to electrocatalytic effects that result in highly sensitive detection.
KeywordsGraphene Reduced graphene oxide Hydrogen peroxide Amperometry Electrical impedance spectroscopy Chronocoulometry Cyclic voltammetry Raman spectroscopy
This work was supported by DFG Research Training Electronic Properties of Carbon Based Nanostructures (GRK 1570).
- 2.Ferrari AC, Bonaccorso F, Fal’ko V, Novoselov KS, Roche S, Bøggild P, Borini S, Koppens FHL, Palermo V, Pugno N, Garrido JA, Sordan R, Bianco A, Ballerini L, Prato M, Lidorikis E, Kivioja J, Marinelli C, Ryhänen T, Morpurgo A, Coleman JN, Nicolosi V, Colombo L, Fert A, Garcia-Hernandez M, Bachtold A, Schneider GF, Guinea F, Dekker C, Barbone M, Sun Z, Galiotis C, Grigorenko AN, Konstantatos G, Kis A, Katsnelson M, Vandersypen L, Loiseau A, Morandi V, Neumaier D, Treossi E, Pellegrini V, Polini M, Tredicucci A, Williams GM, Hong BH, Ahn J-H, Kim JM, Zirath H, van Wees BJ, van der Zant H, Occhipinti L, Matteo AD, Kinloch IA, Seyller T, Quesnel E, Feng X, Teo K, Rupesinghe N, Hakonen P, Neil SRT, Tannock Q, Löfwander T, Kinaret J (2015) Science and technology roadmap for graphene, related two-dimensional crystals, and hybrid systems. Nanoscale 7:4598–4810. doi: 10.1039/C4NR01600A CrossRefGoogle Scholar
- 11.Shang NG, Papakonstantinou P, McMullan M, Chu M, Stamboulis A, Potenza A, Dhesi SS, Marchetto H (2008) Catalyst-free efficient growth, orientation and biosensing properties of multilayer graphene nanoflake films with sharp edge planes. Adv Funct Mater 18:3506–3514. doi: 10.1002/adfm.200800951 CrossRefGoogle Scholar
- 30.Liu S, Tian J, Wang L, Li H, Zhang Y, Sun X (2010) Stable aqueous dispersion of graphene nanosheets: noncovalent functionalization by a polymeric reducing agent and their subsequent decoration with Ag nanoparticles for enzymeless hydrogen peroxide detection. Macromolecules 43:10078–10083. doi: 10.1021/ma102230m CrossRefGoogle Scholar
- 32.Maji SK, Sreejith S, Mandal AK, Ma X, Zhao Y (2014) Immobilizing gold nanoparticles in mesoporous silica covered reduced graphene oxide: a hybrid material for cancer cell detection through hydrogen peroxide sensing. ACS Appl Mater Interfaces 6:13648–13656. doi: 10.1021/am503110s CrossRefGoogle Scholar