Microchimica Acta

, Volume 182, Issue 15–16, pp 2403–2410 | Cite as

Sensing nitric oxide with a carbon nanofiber paste electrode modified with a CTAB and nafion composite

  • Dongyun Zheng
  • Xiaojun LiuEmail author
  • Shanying Zhu
  • Huimin Cao
  • Yaguang Chen
  • Shengshui HuEmail author
Original Paper


We describe an electrochemical sensor for nitric oxide that was obtained by modifying the surface of a nanofiber carbon paste microelectrode with a film composed of hexadecyl trimethylammonium bromide and nafion. The modified microelectrode displays excellent catalytic activity in the electrochemical oxidation of nitric oxide. The mechanism was studied by scanning electron microscopy and cyclic voltammetry. Under optimal conditions, the oxidation peak current at a working voltage of 0.75 V (vs. SCE) is related to the concentration of nitric oxide in the 2 nM to 0.2 mM range, and the detection limit is as low as 2 nM (at an S/N ratio of 3). The sensor was successfully applied to the determination of nitric oxide released from mouse hepatocytes.

Graphical abstract

NO electrochemical sensor based on CTAB-Nafion/CNFPME was fabricated through a simple method and applied to detect NO released from mouse hepatocytes successfully.


Hexadecyl trimethyl ammonium bromide Nafion Carbon nanofibers Carbon paste electrode Nitric oxide Mouse hepatocytes 



This research is supported by the National Nature Science Foundation of China (Nos. 31070885; 61178087), the Fundamental Research Funds for the Central Universities, South-Central University for Nationalities (Nos. CZY14017; CTZ12001), the Scientific Research Team Project of South-central University for Nationalities (No. XTZ09002).

Supplementary material

604_2015_1561_MOESM1_ESM.doc (680 kb)
ESM 1 (DOC 680 kb)


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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  1. 1.College of Biomedical EngineeringSouth-Central University for NationalitiesWuhanChina
  2. 2.Key Laboratory of Brain Cognitive Science, State Ethnic Affairs CommissionSouth-Central University for NationalitiesWuhanChina
  3. 3.College of Chemistry and Molecule ScienceWuhan UniversityWuhanChina

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