Microchimica Acta

, Volume 182, Issue 11–12, pp 2037–2043 | Cite as

Gold nanoparticles-based colorimetric and visual creatinine assay

  • Yi HeEmail author
  • Xianhui Zhang
  • Haili Yu
Original Paper


We demonstrate a selective and sensitive method for determination of creatinine using citrate-stabilized gold nanoparticles (AuNPs) as a colorimetric probe. It is based on a direct cross-linking reaction that occurs between creatinine and AuNPs that causes aggregation of AuNPs and results in a color change from wine red to blue. The absorption peak is shifted from 520 to 670 nm. Under the optimized conditions, the shift in the absorption peak is related the logarithm of the creatinine concentration in the 0.1 to 20 mM range, and the instrumental detection limit (LOD) is 80 μM. This LOD is about one order of magnitude better than that that of the Jaffé method (720 μM). The assay displays good selectivity over interfering substances including various inorganic ions, organic small compounds, proteins, and biothiols. It was successfully employed to the determination of creatinine in spiked human urine.

Graphical Abstract

The colorimetric assay for creatinine uses citrate-stabilized gold nanoparticles (AuNPs) and a direct cross-linking reaction that occurs between creatinine and AuNPs that causes aggregation of AuNPs and results in a color change from wine red to blue.


Creatinine Gold nanoparticles Visual detection Colorimetry Visualization 



The support of this research by the Doctoral Program of Southwest University of Science and Technology (Grant Nos. 14zx7165), Foundation of Science and Technology Department of Sichuan Province (Grant No. 2015JY0053), Teaching Reform Project of Southwest University of Science and Technology (Grant No. 15xn0077), and Undergraduate Innovation Fund Project of Southwest University of Science and Technology (CX15-011) is gratefully acknowledged.

Supplementary material

604_2015_1546_MOESM1_ESM.doc (1.3 mb)
ESM 1 (DOC 1297 kb)


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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  1. 1.School of National Defence Science & TechnologySouthwest University of Science and TechnologyMianyangPeople’s Republic of China

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