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Microchimica Acta

, Volume 182, Issue 9–10, pp 1709–1714 | Cite as

Impedimetric aptamer-based determination of the mold toxin fumonisin B1

  • Xiujuan Chen
  • Yukun Huang
  • Xiaoyuan Ma
  • Fei Jia
  • Xiaofei Guo
  • Zhouping WangEmail author
Original Paper

Abstract

We are presenting an aptasensor for the sensitive determination of fumonisin B1 (FB-1) via electrochemical impedance spectroscopy (EIS) and applying aptamer-based biorecognition. A thiolated aptamer for FB-1 was anchored onto the surface of gold nanoparticles (AuNPs) on a glassy carbon electrode. A significant increase in resistance (Ret) is found on interaction with FB-1 in the 0.1 nM to 100 μM concentration range, and the detection limit is as low as 2 pM. The assay was applied to determine FB-1 in spiked maize samples and gave recovery rates ranging from 91 to 105 %. The results demonstrate this method to present new possibilities in the application of aptamers in food safety analysis.

Graphical abstract

The electrodeposited AuNPs create a nanomaterial platform in situ for anchoring of FB-1 aptamers serving as recognition elements. The change in EIS signals induced by aptamer-target interactions was measured and a significant increase in resistance (Ret) is found on interaction with FB-1 in the 0.1 nM to 100 μM concentration range.

Keywords

Fumonisin Biosensor Electrochemical impedance spectroscopy (EIS) Electrodeposited gold nanoparticles Aptamer 

Notes

Acknowledgments

This work was partly supported by the National S&T Support Program of China (2012BAK08B01), S&T Supporting Project of Jiangsu Province(BE2011621), and JUSRP51309A.

Conflict of interest

The authors declare no competing financial interests.

Supplementary material

604_2015_1492_MOESM1_ESM.doc (127 kb)
ESM 1 (DOC 127 kb)

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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Xiujuan Chen
    • 1
  • Yukun Huang
    • 1
  • Xiaoyuan Ma
    • 1
  • Fei Jia
    • 1
  • Xiaofei Guo
    • 1
  • Zhouping Wang
    • 1
    Email author
  1. 1.State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and TechnologyJiangnan UniversityWuxiChina

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