Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin
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Epitope imprinted polymer nanoparticles (EI-NPs) were prepared by one-pot polymerization of N-isopropylacrylamide in the presence of CdTe quantum dots and an epitope (consisting of amino acids 598 to 609) of human serum albumin (HSA). The resulting EI-NPs exhibit specific recognition ability and enable direct fluorescence quantification of HSA based on a fluorescence turn-on mode. The polymer was characterized by FT-IR, X-ray photoelectron spectroscopy, transmission electron microscopy and dynamic light scattering. The linear calibration graph was obtained in the range of 0.25–5 μmol · mL−1 with the detection limit of 44.3 nmol · mL−1. The EI-NPs were successfully applied to the direct fluorometric quantification of HSA in samples of human serum. Overall, this approach provides a promising tool to design functional fluorescent materials with protein recognition capability and specific applications in proteomics.
KeywordsEpitope imprinting Human serum albumin Quantum dots Turn-on fluorescence Fluorometry
This work was supported by the National Basic Research Program of China (973 Program) (Nos. 2011CB707703 and 2012CB910601) and the National Natural Science Foundation of China (Nos. 21275078 and 21475069).
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