A regenerable piezoelectric immunosensor on the basis of electropolymerized polypyrrole for highly selective detection of Staphylococcal Enterotoxin A in foodstuffs
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Piezoelectric immunosensors have been developed for the determination of trace concentrations of Staphylococcal enterotoxin A (SEA) in foodstuff. Antibodies against SEA were covalently immobilized on an electropolymerized polypyrrole substrate on a gold electrode which warrants increased stability of the biolayer and sensitive detection. The calibration plot is linear in the 1–80 ng∙mL‾1 SEA concentration range, the limit of detection is 0.4 ng∙mL‾1, and the time of analysis is <30 min. The sensor was successfully applied to quantify SEA in chicken meat and milk.
KeywordsPiezoelectric immunosensor Antibodies Immobilization Staphylococcal enterotoxin A Polypyrrole
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