Microwave-assisted synthesis of BSA-modified silver nanoparticles as a selective fluorescent probe for detection and cellular imaging of cadmium(II)
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We have developed a microwave-assisted method for the synthesis of silver nanoparticles (AgNPs) whose surface is modified with bovine serum albumin (BSA). The reaction involves reduction of the BSA-Ag(I) complex by tyrosine in strongly alkaline solution to form BSA-AgNPs. The reaction takes a few minutes only owing to rapid and uniform microwave heating. The modified AgNPs were characterized by UV–vis and fluorescence spectroscopy, transmission electron microscopy and X- ray photoelectron spectroscopy. The BSA-AgNPs are yellow and display luminescence with a maximum at 521 nm if excited at 465 nm. They have a hydrodynamic diameter of 3–5 nm and possess good colloidal stability in the pH 4.6 to 12.0 range. The fluorescence of the BSA-AgNPs is enhanced by Cd(II) ion due to the formation of a stable hybrid conjugate referred to as Cd@BSA-AgNPs. The effect was exploited to quantify Cd(II) in spiked real water samples with a 4.7 nM detection limit, and also to fluorescently image Cd(II) in Hepatoma cells.
KeywordsFluorescence Fluorescent probe Silver nanoparticle BSA Microwave synthesis
This work was financially supported by The Research and Innovation Project for Graduate Students Academic Degree of Colleges and Universities of Jiangsu Province (KYLX_0618), National Found for Fostering Talents of Basic Science (NFFTBS)-Provincial Innovation and Entrepreneurship Training Program for College Students (No.J1030830). We are delighted to acknowledge discussions with colleagues in our research group.
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