Screen-printed biosensor modified with carbon black nanoparticles for the determination of paraoxon based on the inhibition of butyrylcholinesterase
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We have developed a screen-printed electrochemical electrode (SPE) for paraoxon based on its inhibitory effect on the enzyme butyrylcholinesterase (BChE). The electrode was first modified by drop casting with a dispersion of carbon black nanoparticles (CBNPs) in a dimethylformamide-water mixture, and BChE was then immobilized on the surface by cross-linking. The resulting biosensor was exposed to standard solutions of paraoxon, and the enzymatic hydrolysis of butyrylthiocholine over time was determined measuring the enzymatic product thiocholine at a working voltage of +300 mV. The enzyme inhibition is linearly related to the concentration of paraoxon up to 30 μg L−1, and the detection limit is 5 μg L−1. The biosensor is stable for up to 78 days of storage at room temperature under dry conditions. It was applied to determined paraoxon in spiked waste water samples. The results underpin the potential of the use of CBNPs in electrochemical biosensors and also demonstrate that they represent a viable alternative to other carbon nanomaterials such as carbon nanotubes or graphene, and with the advantage of being very affordable.
KeywordsOrganophosphate Butyrylcholinesterase Screen-printed electrode Carbon black nanoparticles Inhibition
This work was supported by National Industria 2015 (MI01_00223) ACQUA-SENSE project and Marie Curie FP7-PEOPLE-2011-IRSES, 294901 “Peptide Nanosensors”. The authors thank Prof. F. Cataldo (Actinium Chemical Research srl) for the CBNPs samples, Tover Italia s.r.l. (Rome) and BASF Italia Divisione Catalizzatori (Rome) for the waste water samples.
- 1.Bergh C, Torgrip R, Ostman C (2010) Simultaneous selective detection of organophosphate and phthalate esters using gas chromatography with positive ion chemical ionization tandem mass spectrometry and its application to indoor air and dust. Rapid Commun Mass Spectrom 24:2859–2867CrossRefGoogle Scholar
- 6.Taleat Z, Khoshroo A, Mazloum-Ardakani M (2014) Screen-printed electrodes for biosensing: a review (2008–2013) Microchim Acta, in press, doi: 10.1007/s00604-014-1181-1
- 32.Arduini F, Palleschi G (2012) Disposable Electrochemical Biosensor Based on Cholinesterase Inhibition with Improved Shelf-Life and Working Stability for Nerve Agent Detection. In: Nikolelis DP (ed) NATO science for peace and security series A: chemistry and biology portable chemical sensors weapons against bioterrorism. Springer, The Netherlands, pp 261–278Google Scholar
- 36.Tang X, Zhang T, Liang B, Han D, Zeng L, Zheng C, Li T, Wei M, Liu A (2014) A. Sensitive electrochemical microbial biosensor for p-nitrophenylorganophosphates based on electrode modified with cell surface-displayed organophosphorus hydrolase and ordered mesopore carbons. Biosens Bioelectron 60:137–142CrossRefGoogle Scholar
- 39.DL 152/2006, 3 April 2006 “Norme in materia ambientale”, http://www.gazzettaufficiale.it/eli/id/2006/04/14/006G0171/sg