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Microchimica Acta

, Volume 182, Issue 1–2, pp 395–400 | Cite as

A colorimetric method for the determination of xanthine based on the aggregation of gold nanoparticles

  • Wendan Pu
  • Huawen ZhaoEmail author
  • Liping Wu
  • Xianying Zhao
Original Paper

Abstract

We describe a highly sensitive method for the determination of traces of xanthine based on the aggregation of citrate-stabilized gold nanoparticles (AuNPs). It is found that, under optimal conditions of pH, the imide group of xanthine is adsorbed on the surface of the AuNPs, thereby displacing citrate ions. This leads to an aggregation of the AuNPs via hydrogen-bond interactions. As a result, the color of the solution changes from red to blue which can be seen with bare eyes and also can be measured by spectrophotometry. The ratio of the absorbances at 630 nm and 520 nm is linearly related to the concentration of xanthine in the 125 nM to 6.0 μM range (r = 0.9988), and the detection limit (3σ/slope) is 23 nM. The method is simple, feasible and fast.

Graphical Abstract

We describe a highly sensitive colorimetric method for the determination of traces of xanthine based on the aggregation of citrate-stabilized gold nanoparticles (AuNPs). The ratio of the absorbances at 630 nm and 520 nm is linearly related to the concentration of xanthine in the 125 nM to 6.0 μM range, and the detection limit is 23 nM. The method is simple, feasible and fast.

Keywords

Xanthine Gold nanoparticles Aggregation Detection 

Notes

Acknowledgments

This work was supported by the Chongqing Natural Science Foundation (CSTC, 2007BB0049) and the National Natural Science Foundation Committee of China.

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Wendan Pu
    • 1
  • Huawen Zhao
    • 1
    Email author
  • Liping Wu
    • 1
  • Xianying Zhao
    • 1
  1. 1.Department of ChemistryThird Military Medical UniversityChongqingPeople’s Republic of China

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