Microchimica Acta

, Volume 181, Issue 13–14, pp 1565–1572 | Cite as

A bare-eye-based lateral flow immunoassay based on the use of gold nanoparticles for simultaneous detection of three pesticides

  • Limin Wang
  • Jia Cai
  • Yulong Wang
  • Qingkui Fang
  • Suyan Wang
  • Qi Cheng
  • Dan Du
  • Yuehe Lin
  • Fengquan Liu
Original Paper


We present a novel lateral flow immunoassay (LFIA) for the simultaneous detection of the pesticides imidacloprid, chlorpyrifos-methyl and isocarbophos based on three competitive immunoreactions. In contrast to previously reported LFIAs, the method is based on the use of four strips. Each has three red channels (three test lines dispensed with different capture reagent) to detect imidacloprid, chlorpyrifos-methyl and isocarbophos respectively. Different channels on each strip are the key to multi-detection, and four strips of LFIA are needed for visual and semi-quantitative read-outs. Under optimized conditions, the LFIA was applied to the determination of three pesticides. The detection time is within 7 min and the detection limits are 50, 100, and 100 μg L−1, respectively. Furthermore, the LFIA was applied to the analysis of spiked Chinese cabbage and soil samples and results were validated by HPLC.


Design of the Lateral Flow Immunoassay. The LFIA made up of four strips (Strip 1 to Strip 4), and each strip dispensed with three kinds of capture antigens on different channels (CH1 to CH3)


Lateral flow immunoassay Simultaneous detection Competitive immunoreactions Bare eye readout 



This work was done at Nanjing Agricultural University and supported by the National High Technology Research and Development Program of China (2012AA101401-1, 2013AA065601), and the Youth Science and Technology Innovation Foundation of NJAU (KJ2012003)


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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Limin Wang
    • 1
    • 2
  • Jia Cai
    • 1
    • 2
  • Yulong Wang
    • 1
    • 2
  • Qingkui Fang
    • 1
    • 2
  • Suyan Wang
    • 1
    • 2
  • Qi Cheng
    • 1
    • 2
  • Dan Du
    • 3
  • Yuehe Lin
    • 3
  • Fengquan Liu
    • 1
    • 2
  1. 1.College of Plant ProtectionNanjing Agricultural UniversityNanjingPeople’s Republic of China
  2. 2.China/Key Laboratory of Integrated Management of Crop Disease and Pests (Nanjing Agricultural University)Ministry of EducationNanjingPeople’s Republic of China
  3. 3.School of Mechanical and Materials EngineeringWashington State UniversityPullmanUSA

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