Microchimica Acta

, Volume 181, Issue 15–16, pp 1823–1832 | Cite as

Magnetic molecularly imprinted polymer nanoparticles for the solid-phase extraction of paracetamol from plasma samples, followed its determination by HPLC

  • Saman Azodi-Deilami
  • Alireza Hassani Najafabadi
  • Ebadullah Asadi
  • Majid Abdouss
  • Davood Kordestani
Original Paper


We are presenting magnetic molecularly imprinted polymer nanoparticles (m-MIPs) for solid-phase extraction and sample clean-up of paracetamol. The m-MIPs were prepared from magnetite (Fe3O4) as the magnetic component, paracetamol as the template, methacrylic acid as a functional monomer, and 2-(methacrylamido) ethyl methacrylate as a cross-linker. The m-MIPs were then characterized by transmission electron microscopy, FT-IR spectroscopy, X-ray diffraction and vibrating sample magnetometry. The m-MIPs were applied to the extraction of paracetamol from human blood plasma samples. Following its elution from the column loaded with the m-MIPs with an acetonitrile-buffer (9:1) mixture, it was submitted to HPLC analysis. Paracetamol can be quantified by this method in the 1 μg L−1 to 300 μg L−1 concentration range. The limit of detection and limit of quantification in plasma samples are 0.17 and 0.4 μg L−1. The preconcentration factor of the m-MIPs is 40. The HPLC method shows good precision (4.5 % at 50 μg L−1 levels) and recoveries (between 83 and 91 %) from spiked plasma samples.


We are presenting magnetic molecularly imprinted polymer nanoparticles (m-MIPs) for solid-phase extraction and sample clean-up of paracetamol. The m-MIPs were applied to the extraction of paracetamol from human blood plasma samples


Molecularly imprinted polymer nanoparticles Magnetic solid phase extraction Human plasma samples Cross-link agent HPLC 

Supplementary material

604_2014_1230_MOESM1_ESM.docx (588 kb)
ESM 1 (DOCX 587 kb)


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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Saman Azodi-Deilami
    • 1
  • Alireza Hassani Najafabadi
    • 1
  • Ebadullah Asadi
    • 1
  • Majid Abdouss
    • 1
  • Davood Kordestani
    • 2
  1. 1.Department of ChemistryAmirkabir University of TechnologyTehranIran
  2. 2.Faculty of Chemistry, Department of Organic ChemistryRazi UniversityKermanshahIran

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