Microchimica Acta

, Volume 181, Issue 11–12, pp 1239–1247 | Cite as

Cytosine-assisted synthesis of gold nanochains and gold nanoflowers for the construction of a microperoxidase-11 based amperometric biosensor for hydrogen peroxide

  • Qian-Li Zhang
  • Dan-Ling Zhou
  • Yong-Fang Li
  • Ai-Jun WangEmail author
  • Su-Fang Qin
  • Jiu-Ju FengEmail author
Original Paper


A simple method was developed for synthesis of network-like gold nanochains and gold nanoflowers in the presence of cytosine by reduction of tetrachloroauric acid with sodium borohydride and ascorbic acid, respectively. The resulting gold nanocrystals were coated with microperoxidase-11 via electrostatic interactions. Electrodes modified with protein-coated gold nanochains or nanoflowers display well-defined and quasireversible redox peaks and enhanced high electrocatalytic activity toward the reduction of H2O2 that is due to direct electron transfer to the protein. The effects were exploited for the amperometric detection of H2O2 with a linear response from 0.5 μM to 0.13 mM (for the gold nanochains) and from1.0 μM to 0.11 mM (for the gold nanoflowers), respectively. The sensor shows lower detection limit and faster response time than sensors based on the use of spherical gold nanoparticles.


A simple method was developed in the synthesis of network-like Au nanochains and Au nanoflowers in the presence of cytosine by reduction of tetrachloroauric acid with sodium borohydride and ascorbic acid, respectively. Thus-prepared Au nanocrystals were further employed for the construction of microperoxidase-11 based biosensors.


Cytosine Gold nanochains Gold nanoflowers Microperoxidase-11 Biosensor 



This work was financially supported by the NSFC (Nos. 21175118 and 21275130) and colleges in Zhejiang province to the young academic leaders of academic climbing project (pd2013055).

Supplementary material

604_2014_1226_MOESM1_ESM.pdf (211 kb)
ESM 1 (PDF 210 kb)


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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  1. 1.College of Chemistry and Life Science, College of Geography and Environmental ScienceZhejiang Normal UniversityJinhuaChina
  2. 2.College of Chemistry and Biological Engineering, Jiangsu Key Laboratory for Environment Functional MaterialsSuzhou University of Science and TechnologySuzhouChina
  3. 3.College of Chemistry and Chemical EngineeringHenan Institute of Science and TechnologyXinxiangChina

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