Cytosine-assisted synthesis of gold nanochains and gold nanoflowers for the construction of a microperoxidase-11 based amperometric biosensor for hydrogen peroxide
- 382 Downloads
A simple method was developed for synthesis of network-like gold nanochains and gold nanoflowers in the presence of cytosine by reduction of tetrachloroauric acid with sodium borohydride and ascorbic acid, respectively. The resulting gold nanocrystals were coated with microperoxidase-11 via electrostatic interactions. Electrodes modified with protein-coated gold nanochains or nanoflowers display well-defined and quasireversible redox peaks and enhanced high electrocatalytic activity toward the reduction of H2O2 that is due to direct electron transfer to the protein. The effects were exploited for the amperometric detection of H2O2 with a linear response from 0.5 μM to 0.13 mM (for the gold nanochains) and from1.0 μM to 0.11 mM (for the gold nanoflowers), respectively. The sensor shows lower detection limit and faster response time than sensors based on the use of spherical gold nanoparticles.
KeywordsCytosine Gold nanochains Gold nanoflowers Microperoxidase-11 Biosensor
This work was financially supported by the NSFC (Nos. 21175118 and 21275130) and colleges in Zhejiang province to the young academic leaders of academic climbing project (pd2013055).
- 4.Gao MR, Zhang SR, Xu YF, Zheng YR, Jiang J, Yu SH (2014) Self-assembled platinum nanochain networks driven by induced magnetic dipoles. Adv Funct Mater. 24:878Google Scholar
- 11.Fang C, Fan Y, Kong JM, Gao ZQ, Balasubramanian N (2008) Preparation of nanochain and nanosphere by self-assembly of gold nanoparticles. Appl Phys Lett 92:236108Google Scholar
- 17.Zhang S, Kou X, Yang Z, Shi Q, Stucky GD, Sun L, Wang J, Yan C (2007) Nanonecklaces assembled from gold rods, spheres, and bipyramids. Chem Commun :1816Google Scholar
- 32.Yarman A, Neumann B, Bosserdt M, Gajovic-Eichelmann N, Scheller FW (2012) Peroxide-dependent analyte conversion by the heme prosthetic group, the heme peptide “microperoxidase-11” and cytochrome c on chitosan capped gold nanoparticles modified electrodes. Biogeosciences 2:189Google Scholar