Glucose biosensor based on a platinum electrode modified with rhodium nanoparticles and with glucose oxidase immobilized on gold nanoparticles
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We have developed an enzymatic glucose biosensor that is based on a flat platinum electrode which was covered with electrophoretically deposited rhodium (Rh) nanoparticles and then sintered to form a large surface area. The biosensor was obtained by depositing glucose oxidase (GOx), Nafion, and gold nanoparticles (AuNPs) on the Rh electrode. The electrical potential and the fractions of Nafion and GOx were optimized. The resulting biosensor has a very high sensitivity (68.1 μA mM−1 cm−2) and good linearity in the range from 0.05 to 15 mM (r = 0.989). The limit of detection is as low as 0.03 mM (at an SNR of 3). The glucose biosensor also is quite selective and is not interfered by electroactive substances including ascorbic acid, uric acid and acetaminophen. The lifespan is up to 90 days. It was applied to the determination of glucose in blood serum, and the results compare very well with those obtained with a clinical analyzer.
KeywordsBlood glucose Biosensor Rh nanoparticle Gold nanoparticle Nafion
The research was supported by National Natural Science Foundation of China (Grant No. 30600143, 81171416), National Key Technology R&D Program of China (No. 2012BAI16B02), Zhejiang Provincial Natural Science Foundation of China (Grant No. Y13H180019).
- 10.Wang J (2001) Glucose biosensors: 40 years of advances and challenges. Electroanalysis 13(12)Google Scholar
- 12.Norouzi P, Faridbod F, Larijani B, Ganjali MR (2010) Glucose biosensor based on MWCNTs-Gold nanoparticles in a Nafion film on the glassy carbon electrode using flow injection FFT continuous cyclic voltammetry. Int J Electrochem Sci 5:1213–1224Google Scholar
- 18.Soukup J, Polan V, Kotzian P, Kalcher K, Vytřas K (2011) Rhodium and its compounds in amperometric biosensors based on redox enzymes. Int J Electrochem Sci 6:231–239Google Scholar
- 25.Long NN, Van Vu L, Kiem CD, Cong Doanh S, Thi Nguyet C, Thi Hang P, Duy Thien N, Quynh LM (2009) Synthesis and optical properties of colloidal gold nanoparticles. J Physics Conf Ser :2026Google Scholar
- 34.Shi X, Gu W, Li B, Chen N, Zhao K, Xian Y (2013) Enzymatic biosensors based on the use of metal oxide nanoparticles. Microchimica Acta:1–22Google Scholar