Glucose biosensor based on glucose oxidase immobilized on unhybridized titanium dioxide nanotube arrays
- 1.4k Downloads
A glucose biosensor has been fabricated by immobilizing glucose oxidase (GOx) on unhybridized titanium dioxide nanotube arrays using an optimized cross-linking technique. The TiO2 nanotube arrays were synthesized directly on a titanium substrate by anodic oxidation. The structure and morphology of electrode material were characterized by X-ray diffraction and scanning electron microscopy. The electrochemical performances of the glucose biosensor were conducted by cyclic voltammetry and chronoamperometry measurements. It gives a linear response to glucose in the 0.05 to 0.65 mM concentration range, with a correlation coefficient of 0.9981, a sensitivity of 199.6 μA mM−1 cm−2, and a detection limit as low as 3.8 µM. This glucose biosensor exhibited high selectivity for glucose determination in the presence of ascorbic acid, sucrose and other common interfering substances. This glucose biosensor also performed good reproducibility and long-time storage stability. This optimized cross-linking technique could open a new avenue for other enzyme biosensors fabrication.
KeywordsTitanium dioxide Nanotube arrays Glucose oxidase Biosensor Cross-linking
The work was supported by National Natural Science Foundation of China (No. 21373047 and 20871029), Research Fund for the Doctoral Program of Higher Education of China (No. 200802861071), Program for New Century Excellent Talents in University of the State Ministry of Education (No. NCET-08-0119), Science & Technology Program of Suzhou City (No. SYG201017, ZXG2012026, SYN201208) and the Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University (No. 2011E17).