Microchimica Acta

, Volume 181, Issue 3–4, pp 295–301 | Cite as

Peroxidase-like activity of magnetoferritin

  • Lucia Melnikova
  • Kristyna Pospiskova
  • Zuzana Mitroova
  • Peter Kopcansky
  • Ivo Safarik
Original Paper
First Online:
Received:
Accepted:

Abstract

Magnetoferritin is a spherical biomacromolecule with a diameter of about 12 nm. It consists of a protein shell composed of apoferritin that is surrounding magnetic nanoparticles of magnetite (Fe3O4) or maghemite (γ-Fe2O3). Magnetoferritins with various iron content (loading factor) were synthetically prepared and their peroxidase-like activities studied via the oxidation of the chromogenic substrate N,N-diethyl-p-phenylenediamine sulfate by hydrogen peroxide to give a purple product with an absorption maximum at 551 nm. Magnetoferritin with higher loading factor exhibits a higher peroxidase-like activity. The catalytic activity was successfully applied to the determination of hydrogen peroxide in the 5.8 to 88.2 mM concentration range.

Figure

Change of substrate colour in the presence of hydrogen peroxide due to the peroxidase-like activity of magnetoferritin.

Keywords

Magnetoferritin Magnetic nanoparticles Peroxidase-like activity Hydrogen peroxide Oxidative stress 
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Notes

Acknowledgments

This work was supported by the Slovak Scientific Grant Agency VEGA (project No. 2/0045/13), by the project APVV 0171–10 (METAMYLC) and by the European Structural Funds, Projects No. 26110230061 (NANOKOP) and 26220120021. This work was also supported by the Ministry of Education of the Czech Republic (project No. LD13021).

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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Lucia Melnikova
    • 1
  • Kristyna Pospiskova
    • 2
    • 4
  • Zuzana Mitroova
    • 1
  • Peter Kopcansky
    • 1
  • Ivo Safarik
    • 3
    • 4
  1. 1.Institute of Experimental Physics, Slovakian Acad. ScienceKosiceSlovakia
  2. 2.Department of Biochemistry, Faculty of SciencePalacky UniversityOlomoucCzech Republic
  3. 3.Department of NanobiotechnologyInstitute of Nanobiology and Structural Biology of GCRCCeske BudejoviceCzech Republic
  4. 4.Regional Centre of Advanced Technologies and MaterialsPalacky UniversityOlomoucCzech Republic

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