Microchimica Acta

, Volume 180, Issue 15–16, pp 1517–1522 | Cite as

Protein-directed in situ synthesis of platinum nanoparticles with superior peroxidase-like activity, and their use for photometric determination of hydrogen peroxide

  • Lijian Chen
  • Nan Wang
  • Xindong Wang
  • Shiyun AiEmail author
Short Communication


Platinum nanoparticles (Pt-NPs) with sizes in the range from 10 to 30 nm were synthesized using protein-directed one-pot reduction. The model globular protein bovine serum albumin (BSA) was exploited as the template, and the resulting BSA/Pt-NPs were studied by transmission electron microscopy, energy dispersive X-ray spectroscopy, and resonance Rayleigh scattering spectroscopy. The modified nanoparticles display a peroxidase-like activity that was exploited in a rapid method for the colorimetric determination of hydrogen peroxide which can be detected in the 50 μM to 3 mM concentration range. The limit of detection is 7.9 μM, and the lowest concentration that can be visually detected is 200 μM.


Pt-NPs were synthesized using BSA-directed one-pot reduction and BSA/Pt-NPs composite can effectively catalyze the oxidation of TMB producing blue solution in the presence of H2O2.


Bovine serum albumin In situ synthesis Platinum nanoparticles Colorimetric detection 



This work was financially supported by the National Natural Science Foundation of China (No.21075078, 21105056) and the Natural Science Foundation of Shandong province, China (No.ZR2010BM005, ZR2011BQ001).

Supplementary material

604_2013_1068_MOESM1_ESM.doc (982 kb)
ESM 1 (DOC 982 kb)


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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Lijian Chen
    • 1
  • Nan Wang
    • 1
  • Xindong Wang
    • 1
  • Shiyun Ai
    • 1
    Email author
  1. 1.College of Chemistry and Material ScienceShandong Agricultural UniversityTaianPeople’s Republic of China

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