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Microchimica Acta

, Volume 180, Issue 11–12, pp 1005–1011 | Cite as

Electrochemical sensor based on a carbon nanotube-modified imprinted sol–gel for selective and sensitive determination of ß2-agonists

  • Wei Xu
  • Ping Liu
  • Chunhui Guo
  • Chao Dong
  • Xiuhua Zhang
  • Shengfu Wang
Original Paper

Abstract

We describe a molecularly imprinted electrochemical sensor for selective and sensitive determination of β2-agonists. It is making use of a combination of single-wall carbon nanotubes (SWNTs) with a molecularly imprinted sol–gel. The SWNTs were introduced in order to enhance electron transport and sensitivity. The imprinted sol–gel film with its specific binding sites acts as a selective recognition element and as a preconcentrator for β2-agonists. The morphology of the imprinted film was characterized by scanning electron microscopy. The optimized sensor displays high sensitivity and excellent selectivity for the β2-agonists as shown for their determination in human serum samples.

Figure

A molecularly imprinted electrochemical sensor was constructed for selective and sensitive determination of β2-agonists. The optimized sensor displays high sensitivity and excellent selectivity for the β2-agonists as shown for their determination in human serum samples.

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Keywords

Molecularly imprinted polymer Single-wall carbon nanotubes Sol–gel β2-agonists 

Notes

Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 21075029), the Natural Science Fund for Creative Research Groups of Hubei Province of China (No. 2011CDA111), and the Program for Excellent Youth Scholars of Innovative Research Team by Hubei Provincial Department of Education (T201101).

Supplementary material

604_2013_1020_MOESM1_ESM.doc (3.4 mb)
ESM 1 (DOC 3458 kb)

References

  1. 1.
    Virant FS (1997) In: Weiler JM (ed) Allergic and respiratory disease in sports medicine. Marcel Dekker Inc, New York, pp 65–66Google Scholar
  2. 2.
    Mitchell GA, Dunnavan G (1998) Illegal use of beta-adrenergic agonists in the United States. J Anim Sci 76:208Google Scholar
  3. 3.
    Martinez NJF (1990) Food poisoning related to consumption of illicit β-agonist in liver. Lancet 336:1311CrossRefGoogle Scholar
  4. 4.
    Sirichai S, Khanatharana P (2008) Rapid analysis of clenbuterol, salbutamol, procaterol, and fenoterol in pharmaceuticals and human urine by capillary electrophoresis. Talanta 76:1194CrossRefGoogle Scholar
  5. 5.
    Esquisabel A, Herandez RM, Gascon AR, Igartua M, Calvo B (1997) Determination of Salbutamol enantiomers by high performance capillary electrophoresis and its applicationto dissolution assays. J Pharm Biomed Anal 16:357CrossRefGoogle Scholar
  6. 6.
    Lau JHW, Khoo CS, Murby JE (2004) Determination of clenbuterol, salbutamol, and cimaterol in bovine retina by electrospray ionization-liquid chromatography-tandem mass spectrometry. J AOAC Int 87:31Google Scholar
  7. 7.
    Saleh MI, Koh YM, Tan SC, Aishah AL (2000) Clean-up, detection and determination of salbutamol in human urine and serum. Analyst 125:1569CrossRefGoogle Scholar
  8. 8.
    Black SB, Hansson RC (1999) Determination of salbutamol and detection of other β-agonists in human postmortem whole blood and urine by GC-MS-SIM. J Anal Toxicol 23:113CrossRefGoogle Scholar
  9. 9.
    Couper FJ, Drummer OH (1996) Gas chromatographic-mass spectrometric determination of β2-agonists in postmortem blood: application in forensic medicine. J Chromatogr B 685:265CrossRefGoogle Scholar
  10. 10.
    Deveaux M, Kintz P, Goulle JP, Bessard J, Pépin G, Gosset D (2000) The hair analysis proficiency testing program of the French Society of Analytical Toxicology. Forensic Sci Int 107:389CrossRefGoogle Scholar
  11. 11.
    Basavaiah K, Prameela HC (2003) Three useful bromimetric methods for the determination of salbutamol sulfate. Anal Bioanal Chem 376:879CrossRefGoogle Scholar
  12. 12.
    Mohamed GG, Khalil SM, Zayed MA, Ei-Hamid El-Shall MA (2002) 2,6-Dichloroquinone chlorimide and 7,7,8,8-tetracyanoquinodimethane reagents for the spectrophotometric determination of salbutamol in pure and dosage forms. J Pharm Biomed Anal 28:1127CrossRefGoogle Scholar
  13. 13.
    Loss JR II, Orzechowski RF, Hock RS (2000) Measurement of albuterol in guinea pig serum by high performance liquid chromatography with fluorescence detection. Biomed Chromatogr 14:1CrossRefGoogle Scholar
  14. 14.
    Sheu SY, Lei YC, Tai YT, Chang TH, Kuo TF (2009) Screening of salbutamol residues in swine meat and animal feed by an enzyme immunoassay in Taiwan. Anal Chim Acta 654:148CrossRefGoogle Scholar
  15. 15.
    Ventura R, González G, Smeyers MT, Torre R, Segura J (1998) Screening procedure for β-adrenergic drugs in sports drug testing by immunological methods. J Anal Toxicol 22:127CrossRefGoogle Scholar
  16. 16.
    Wang JP, Pan MF, Fang GZ, Wang S (2009) Preparation of a novel molecularly imprinted polymer by a sol–gel process for on-line solid-phase extraction coupled with high performance liquid chromatography to detect trace enrofloxacin in fish and chicken samples. Microchim Acta 166:295CrossRefGoogle Scholar
  17. 17.
    Xie CG, Gao S, Guo QB, Xu K (2010) Electrochemical sensor for 2,4-dichlorophenoxy acetic acid using molecularly imprinted polypyrrole membrane as recognition element. Microchim Acta 169:145CrossRefGoogle Scholar
  18. 18.
    Lin CI, Joseph AK, Chang CK, Wang YC, Lee YD (2003) Synthesis of molecular imprinted organic–inorganic hybrid polymer binding caffeine. Anal Chim Acta 481:175CrossRefGoogle Scholar
  19. 19.
    Umpleby RJ II, Baxter SC, Rampey AM, Rushton GT, Chen Y, Shimizu KD (2004) Characterization of the heterogeneous binding site affinity distributions in molecularly imprinted polymers. J Chromatogr B 804:141CrossRefGoogle Scholar
  20. 20.
    Holthoff EL, Bright FV (2007) Molecularly templated materials in chemical sensing. Anal Chim Acta 594:147CrossRefGoogle Scholar
  21. 21.
    Hoshina K, Horiyama S, Matsunaga H, Haginaka J (2009) Molecularly imprinted polymers for simultaneous determination of antiepileptics in river water samples by liquid chromatography–tandem mass spectrometry. J Chromatogr A 1216:4957CrossRefGoogle Scholar
  22. 22.
    Kala R, Gladis JM, Rao TP (2004) Preconcentrative separation of erbium from Y, Dy, Ho, Tb and Tm by using ion imprinted polymer particles via solid phase extraction. Anal Chim Acta 518:143CrossRefGoogle Scholar
  23. 23.
    Tan J, Wang HF, Yan XP (2009) A fluorescent sensor array based on ion imprinted mesoporous silica. Biosens Bioelectron 24:3316CrossRefGoogle Scholar
  24. 24.
    Marx S, Zaltsman A, Turyan I (2004) Parathion sensor based on molecularly imprinted sol–gel films. Anal Chem 76:120CrossRefGoogle Scholar
  25. 25.
    Lakshmi D, Bossi A, Whitcombe MJ, Chianella I, Fowler SA, Subrahmanyam S, Piletska EV, Piletsky SA (2009) Electrochemical sensor for catechol and dopamine based on a catalytic molecularly imprinted polymer-conducting polymer hybrid recognition element. Anal Chem 81:3576CrossRefGoogle Scholar
  26. 26.
    Feng L, Liu YJ, Tan YY, Hu JM (2004) Biosensor for the determination of sorbitol based on molecularly imprinted electrosynthesized polymers. Biosens Bioelectron 19:1513CrossRefGoogle Scholar
  27. 27.
    Huang CY, Tsai TC, Thomas JL, Lee MH, Liu BD, Lin HY (2009) Urinalysis with molecularly imprinted poly(ethylene-co-vinyl alcohol) potentiostat sensors. Biosens Bioelectron 24:2611CrossRefGoogle Scholar
  28. 28.
    Zhou HJ, Zhang ZJ, He DY, Xiong Y (2005) Flow through chemiluminescence sensor using molecularly imprinted polymer as recognition elements for detection of salbutamol. Sens Actuators B 107:798CrossRefGoogle Scholar
  29. 29.
    Zhao C, Jin GP, Chen LL, Li Y, Yu B (2011) Preparation of molecular imprinted film based on chitosan/nafion/nano-silver/poly quercetin for clenbuterol sensing. Food Chem 129:595CrossRefGoogle Scholar
  30. 30.
    Andrea P, Miroslav S, Silvia S, Stanislav M (2001) A solid binding matrix/molecularly imprinted polymer-based sensor system for the determination of clenbuterol in bovine liver using differential-pulse voltammetry. Sens Actuators B 76:286CrossRefGoogle Scholar
  31. 31.
    Liang RN, Gao Q, Qin W (2012) Potentiometric sensor based on molecularly imprinted polymers for rapid determination of clenbuterol in pig urine. Chin J Anal Chem 40:354CrossRefGoogle Scholar
  32. 32.
    Mi Q, Wang ZW, Chai CY, Zhang J, Zhao B, Chen CY (2011) Multilayer structured immunosensor based on a glassy carbon electrode modified with multi-wall carbon nanotubes, polythionine, and gold nanoparticles. Microchim Acta 173:459CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Wei Xu
    • 1
  • Ping Liu
    • 1
  • Chunhui Guo
    • 1
  • Chao Dong
    • 1
  • Xiuhua Zhang
    • 1
  • Shengfu Wang
    • 1
  1. 1.Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials & Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional MoleculesHubei UniversityWuhanChina

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