Microchimica Acta

, Volume 180, Issue 9–10, pp 829–835 | Cite as

Fluorescent assay for oxytetracycline based on a long-chain aptamer assembled onto reduced graphene oxide

  • Huimin Zhao
  • Sheng Gao
  • Meng Liu
  • Yangyang Chang
  • Xinfei Fan
  • Xie Quan
Original Paper


We report on a fluorescent assay for oxytetracycline (OTC) using a fluorescein-labeled long-chain aptamer assembled onto reduced graphene oxide (rGO). The π-π stacking interaction between aptamer and rGO causes the fluorescence of the label to be almost completely quenched via energy transfer so that the system has very low background fluorescence. The addition of OTC leads to the formation of G-quadruplex OTC complexes and prevents the adsorption of labeled aptamer on the surface of rGO. As a result, fluorescence is restored, and this effect allows for a quantitative assay of OTC over the 0.1–2 μM concentration range and with a detection limit of 10 nM. This method is simple, rapid, selective and sensitive. It may be applied to other small molecule analytes by applying appropriate aptamers.


A simple and sensitive fluorescent assay for oxytetracycline detection based on the different interaction intensity of fluorescein-labeled long-chain aptamer, G-quadruplex-OTC complex with reduced graphene oxide was designed.


Long-chain aptamer Oxytetracycline Fluorescent assay Graphene 



This work was financially supported by the National Natural Science Foundation of China (No. 21277016).

Supplementary material

604_2013_1006_MOESM1_ESM.doc (1.2 mb)
ESM 1 (DOC 1241 kb)


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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Huimin Zhao
    • 1
  • Sheng Gao
    • 1
  • Meng Liu
    • 1
  • Yangyang Chang
    • 1
  • Xinfei Fan
    • 1
  • Xie Quan
    • 1
  1. 1.Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and TechnologyDalian University of TechnologyDalianChina

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