Rapid and sensitive detection of bacteria via platinum-labeled antibodies and on-particle ionization and enrichment prior to MALDI-TOF mass spectrometry
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We introduce a rapid and sensitive approach to study the interactions of an affinity probe with the bacterial wall. Immunoglobulin was immobilized on platinum nanoparticles, and the resulting probe nanoparticles bind to bacterial walls as confirmed by transmission electron microscopy. A MALDI-MS assay was developed that can detect ~105 cfu mL−1 of S. marcescens and E. coli. This approach enables simple, rapid and straightforward detection of bacterial proteins, with high resolution and sensitivity, and without the requirement for tedious washing/separation steps.
KeywordsIgG-Pt NPs MALDI-MS S. marcescens E. coli
This work was supported by the National Science Council, Taiwan under the grant NSC: 99-2811-M-110-031. Authors would like to acknowledge Mr. Kuo-Chiang Wang (NSC Expensive Instrumental Center, National Sun Yat-Sen University) for technical assistance for obtaining the TEM images and Mr. Bo-Sugm Wu (Department of Chemistry, National Sun Yat-Sen University) for assistance in revision the Figure quality.
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