Microchimica Acta

, Volume 180, Issue 5–6, pp 485–492 | Cite as

Rapid and sensitive detection of bacteria via platinum-labeled antibodies and on-particle ionization and enrichment prior to MALDI-TOF mass spectrometry

Original Paper

Abstract

We introduce a rapid and sensitive approach to study the interactions of an affinity probe with the bacterial wall. Immunoglobulin was immobilized on platinum nanoparticles, and the resulting probe nanoparticles bind to bacterial walls as confirmed by transmission electron microscopy. A MALDI-MS assay was developed that can detect ~105 cfu mL−1 of S. marcescens and E. coli. This approach enables simple, rapid and straightforward detection of bacterial proteins, with high resolution and sensitivity, and without the requirement for tedious washing/separation steps.

Figure

Antobody IgG treated Pt NPs are successfully implemented to bind the cell surfaces of target bacteria. The current bio-analytical technique allows simple, rapid and straightforward identification of bacteria. The obtained results proved that IgG modified platinum nanoparticle strategy was also capable to enhance the protein peaks with high signal intensity and resolution.

Keywords

IgG-Pt NPs MALDI-MS S. marcescens E. coli 

Notes

Acknowledgements

This work was supported by the National Science Council, Taiwan under the grant NSC: 99-2811-M-110-031. Authors would like to acknowledge Mr. Kuo-Chiang Wang (NSC Expensive Instrumental Center, National Sun Yat-Sen University) for technical assistance for obtaining the TEM images and Mr. Bo-Sugm Wu (Department of Chemistry, National Sun Yat-Sen University) for assistance in revision the Figure quality.

Supplementary material

604_2013_951_MOESM1_ESM.ppt (343 kb)
ESM 1 (PPT 343 kb)

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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  1. 1.Department of ChemistryNational Sun Yat-Sen UniversityKaohsiungTaiwan
  2. 2.Center for Nanoscience and NanotechnologyNational Sun Yat-Sen UniversityKaohsiungTaiwan
  3. 3.Doctoral Degree Program in Marine BiotechnologyNational Sun Yat-Sen UniversityKaohsiungTaiwan
  4. 4.School of Pharmacy, College of PharmacyKaohsiung Medical UniversityKaohsiungTaiwan
  5. 5.Department of Biological Sciences, Faculty of Agriculture, Science and TechnologyNorth-West University, Mafikeng CampusMmabathoSouth Africa

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