Microchimica Acta

, Volume 180, Issue 3–4, pp 311–318 | Cite as

Magnetic particles–based biosensor for biogenic amines using an optical oxygen sensor as a transducer

  • Kristyna Pospiskova
  • Ivo Safarik
  • Marek Sebela
  • Gabriela Kuncova
Short Communication

Abstract

We have developed a fibre optic biosensor with incorporated magnetic microparticles for the determination of biogenic amines. The enzyme diamine oxidase from Pisum sativum was immobilized either on chitosan-coated magnetic microparticles or on commercial microbeads modified with a ferrofluid. Both the immobilized enzyme and the ruthenium complex were incorporated into a UV-cured inorganic–organic polymer composite and deposited on a lens that was connected, by optical fibres, to an electro-optical detector. The enzyme catalyzes the oxidation of amines under consumption of oxygen. The latter was determined by measuring the quenched fluorescence lifetime of the ruthenium complex. The limits of detection for the biogenic amines putrescine and cadaverine are 25–30 μmol L−1, and responses are linear up to a concentration of 1 mmol L−1.

Figure

Response (fluorescence lifetime) of a novel optical biosensor for biogenic amines (putrescine, cadaverine) determination based on Pisum sativum diamine oxidase immobilized on magnetically responsive chitosan microparticles with entrapped magnetite encapsulated in inorganic–organic polymer ORMOCER® together with ruthenium complex.

Keywords

Magnetic carriers Optical fibre biosensor Diamine oxidase Fluorescence quenching Ruthenium complex 

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Copyright information

© Springer-Verlag Wien 2012

Authors and Affiliations

  • Kristyna Pospiskova
    • 1
  • Ivo Safarik
    • 2
    • 3
  • Marek Sebela
    • 1
    • 4
  • Gabriela Kuncova
    • 5
  1. 1.Department of Biochemistry, Faculty of SciencePalacky UniversityOlomoucCzech Republic
  2. 2.Department of NanobiotechnologyInstitute of Nanobiology and Structural Biology of GCRC, Academy of SciencesCeske BudejoviceCzech Republic
  3. 3.Regional Centre of Advanced Technologies and MaterialsPalacky UniversityOlomoucCzech Republic
  4. 4.Department of Protein Biochemistry and Proteomics, Centre of the Region Hana for Biotechnological and Agricultural Research, Faculty of SciencePalacky UniversityOlomoucCzech Republic
  5. 5.Institute of Chemical Process Fundamentals, Academy of SciencesPrague 6Czech Republic

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