Microchimica Acta

, Volume 180, Issue 1–2, pp 59–64 | Cite as

Fluorometric sensing of DNA using curcumin encapsulated in nanoparticle-assembled microcapsules prepared from poly(diallylammonium chloride-co-sulfur dioxide)

Original Paper
First Online:
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Accepted:

Abstract

We report on the synthesis of microcapsules (MCs) containing self-assembled nanoparticles formed from poly[diallylammonium chloride-co-(sulfur dioxide)] in the presence of citrate and silica sol nanoparticles. The MCs are spherical, and SEM and optical microscopy reveal them to have micrometer size. The fluorescent probe curcumin was encapsulated in the MCs and found to be located in the shell. The fluorescence of curcumin in the MCs is altered depending on their microenvironment. Effects of pH and ammonia on the fluorescence of curcumin in the MCs also were studied. The brightness of the probe in the MCs increases on addition of DNA. The effect was used to determine DNA from fish sperm by fluorometry. The association constant (K) is 4 000 mL.g−1, and the number of binding sites is ~1.0.

Figure

Synthesis of microcapsule containing self-assembled nanoparticles by using Poly(diallyl ammonium chloride-co-SO2 in the presence of trisodium citrate and silica sol nanoparticles is achieved. Change in the photo-physical properties of the probe molecule suggests a different environment inside the microcapsule. The curcumin encapsulated microcapsules strongly bind to DNA by increasing the brightness with an association constant of 3.98 × 103 mL/g. DNA could be successfully determined using the prepared curcumin encapsulated microcapsules.

Keywords

PDAAC-SO2 Curcumin Nanoparticles Microcapsules DNA sensor 
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Notes

Acknowledgement

Financial support provided by American University of Beirut, Lebanon and Lebanese National Council of Scientific Research (LNCSR), Lebanon to carry out this work is greatly acknowledged.

Supplementary material

604_2012_903_MOESM1_ESM.docx (653 kb)
ESM 1 (DOCX 652 kb)

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Copyright information

© Springer-Verlag Wien 2012

Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of Arts and SciencesAmerican University of BeirutRiad El Solh, BeirutLebanon

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