Advertisement

Microchimica Acta

, Volume 178, Issue 3–4, pp 373–379 | Cite as

Silver nanoparticles plasmon resonance-based method for the determination of uric acid in human plasma and urine samples

  • Mohammad AmjadiEmail author
  • Elaheh Rahimpour
Original Paper

Abstract

We have developed a simple and sensitive colorimetric procedure for the quantification of trace amounts of uric acid. It is based on the finding that uric acid in a medium containing ammonia and sodium hydroxide at 65 °C can reduce silver ions to form yellow silver nanoparticles (Ag NPs). These are stabilized in solution by using poly(vinyl alcohol) as a capping agent. The yellow color of the solution that results from the localized surface plasmon resonance of Ag NPs can be observed by the bare eye. The absorbance at 415 nm is proportional to the concentration of uric acid which therefore can be determined quantitatively. The calibration curve is linear in the concentration range from 10 to 200 nM, with a limit of detection of 3.3 nM. The method was successfully applied to the determination of uric acid in human plasma and urine samples.

Figure

A colorimetric procedure has been developed for the determination of uric acid based on the formation of yellow Ag NPs by the reaction of uric acid with silver ions in a medium containing ammonia and sodium hydroxide at 65 °C.

Keywords

Uric acid Silver nanoparticles Localized surface plasmon resonance Colorimetric method 

Supplementary material

604_2012_849_MOESM1_ESM.doc (224 kb)
ESM 1 (DOC 224 kb)

References

  1. 1.
    Marshall WJ, Bangert SK (2004) Clinical chemistry, 5th edn. Mosby, Edinburg, pp 283–289Google Scholar
  2. 2.
    Bravo R, Hsueh C, Jaramillo A, Brajter-Toth A (1998) Possibilities and limitations in miniaturized sensor design for uric acid. Analyst 123:1625–1630CrossRefGoogle Scholar
  3. 3.
    Zhao Y, Yang X, Lu W, Liao H, Lia F (2009) Uricase based methods for determination of uric acid in serum. Microchim Acta 164:1–6CrossRefGoogle Scholar
  4. 4.
    Lakshmi D, Whitcombe MJ, Davis F, Sharma PS, Prasad BB (2011) Electrochemical detection of uric acid in mixed and clinical sample: a review. Electroanalysis 23:305–320CrossRefGoogle Scholar
  5. 5.
    Zare HR, Rajabzadeh N, Nasirizadeh N, Ardakani MM (2006) Voltammetric studies of an oracet blue modified glassy carbon electrode and its application for the simultaneous determination of dopamine, ascorbic acid and uric acid. J Electroanal Chem 589:60–69CrossRefGoogle Scholar
  6. 6.
    Nassef HM, Radi AE (2007) Simultaneous detection of ascorbate and uric acid using a selectively catalytic surface. Anal Chim Acta 583:182–189CrossRefGoogle Scholar
  7. 7.
    Popa E, Yoshinobu K, Tryk DA, Fujishima A (2000) Selective voltammetric and amperometric detection of uric acid with oxidized diamond film electrodes. Anal Chem 72:1724–1727CrossRefGoogle Scholar
  8. 8.
    Alia SMU, Alvia NH, Ibupotoa Z, Nura O, Willandera M, Danielssonb B (2011) Selective potentiometric determination of uric acid with uricase immobilized on ZnO nanowires. Sensors Actuators B 152:241–247CrossRefGoogle Scholar
  9. 9.
    Fang B, Feng Y, Wang G, Zhang C, Gu A, Liu M (2011) A uric acid sensor based on electrodeposition of nickel hexacyanoferrate nanoparticles on an electrode modified with multi-walled carbon nanotubes. Microchim Acta 173:27–32CrossRefGoogle Scholar
  10. 10.
    Wang Y (2011) The electrochemistry of uric acid at a gold electrode modified with L-cysteine, and its application to sensing uric urine. Microchim Acta 172:419–424CrossRefGoogle Scholar
  11. 11.
    Habibi B, Pezhhan H, Pournaghi-Azar MH (2010) Voltammetric and amperometric determination of uric acid at a carbon-ceramic electrode modified with multi walled carbon nanotubes. Microchim Acta 169:313–320CrossRefGoogle Scholar
  12. 12.
    Wang G, Sun J, Zhang W, Jiao S, Fang B (2009) Simultaneous determination of dopamine, uric acid and ascorbic acid with LaFeO3 nanoparticles modified electrode. Microchim Acta 164:357–362CrossRefGoogle Scholar
  13. 13.
    Noroozifar M, Khorasani-Motlagh M, Taheri A (2010) Preparation of silver hexacyanoferrate nanoparticles and its application for the simultaneous determination of ascorbic acid, dopamine and uric acid. Talanta 80:1657–1664CrossRefGoogle Scholar
  14. 14.
    Kannan P, Abraham John S (2009) Determination of nanomolar uric and ascorbic acids using enlarged gold nanoparticles modified electrode. Anal Biochem 386:65–72CrossRefGoogle Scholar
  15. 15.
    Matos RC, Augelli MA, Lago CL, Angnes L (2000) Flow injection analysis amperometric determination of ascorbic and uric acid in urine using arrays of gold microelectrodes modified by electrodeposition of palladium. Anal Chim Acta 404:151–157CrossRefGoogle Scholar
  16. 16.
    Yamamoto K, Ohgaru T, Torimura M, Kinoshita H, Kano K, Ikeda T (2000) Highly-sensitive flow injection determination of hydrogen peroxide with a peroxidase-immobilized electrode and its application to clinical chemistry. Anal Chim Acta 406:201–207CrossRefGoogle Scholar
  17. 17.
    Araujo AN, Catita JAM, Lima JLFC (1998) Kinetic determination of uric acid in urine based on single-line flow-system with multi-site detection. Anal Sci 14:809–813CrossRefGoogle Scholar
  18. 18.
    Moghadama MR, Dadfarnia S, Haji Shabani AM, Shahbazikhah P (2011) Chemometric-assisted kinetic–spectrophotometric method for simultaneous determination of ascorbic acid, uric acid, and dopamine. Anal Biochem 410:289–295CrossRefGoogle Scholar
  19. 19.
    Hong HC, Huang HJ (2003) Flow injection analysis of uric acid with a uricase- and horseradish peroxidase-coupled Sepharose column based luminol chemiluminescence system. Anal Chim Acta 499:41–46CrossRefGoogle Scholar
  20. 20.
    Song ZH, Hou S (2002) Chemiluminescence assay for uric acid in human serum and urine using flow injection with immobilized reagent technology. Anal Bioanal Chem 372:327–332CrossRefGoogle Scholar
  21. 21.
    Masaaki Y, Akio S (2005) Fluorometric determination of uric acids by flow injection analysis using immobilized uricase and horseradish peroxidase column. Bunseki Kagaku 54:891–896CrossRefGoogle Scholar
  22. 22.
    Dai X, Fang X, Zhang C, Xu R, Xu B (2007) Determination of serum uric acid using high-performance liquid chromatography (HPLC)/isotope dilution mass spectrometry (ID-MS) as a candidate reference method. J Chromatogr B 85:287–295CrossRefGoogle Scholar
  23. 23.
    Cooper N, Khosravan R, Erdmann C, Fiene LJW (2006) Quantification of uric acid, xanthine and hypoxanthine in human serum by HPLC for pharmacodynamic studies. J Chromatogr B 837:1–10CrossRefGoogle Scholar
  24. 24.
    Felice LJ, Dombrovskis D, Lafond E, Bartges J, Osborne CA (2009) Determination of uric acid in canine serum and urine by high performance liquid chromatography. Vet Clin Pathol 19:86–89CrossRefGoogle Scholar
  25. 25.
    Jiye A, Trygg J, Gullberg J, Annika IJ, Jonsson P, Antti H, Marklund SL, Moritz T (2005) Extraction and GC/MS analysis of the human blood plasma metabolome. Anal Chem 77:8086–8094CrossRefGoogle Scholar
  26. 26.
    Zhao S, Lan X, Liu YM (2009) Gold nanoparticles-enhanced capillary electrophoresis-chemiluminescence assay of trace uric acid. Electrophoresis 30:2676–2680CrossRefGoogle Scholar
  27. 27.
    Wang J, Chatrathi MP, Tian BM, Polsky R (2000) Micro-fabricated electrophoresis chips for simultaneous bioassays of glucose, uric acid, ascorbic acid. Anal Chem 72:2514–2518CrossRefGoogle Scholar
  28. 28.
    Jain PR, Huang X, El-Sayed IH, El-Sayed MA (2008) Noble metals on the nanoscale: optical and photothermal properties and some applications in imaging, sensing, biology and medicine. Acc Chem Res 41:1578–1586CrossRefGoogle Scholar
  29. 29.
    Cobley CM, Skrabalak SE, Campbell DJ, Xia Y (2009) Shape-controlled synthesis of silver nanoparticles for plasmonic and sensing applications. Plasmonics 4:171–179CrossRefGoogle Scholar
  30. 30.
    Mock JJ, Barbic M, Smith DR, Schultz DA, Schultz S (2002) Shape effects in plasmon resonance of individual silver nanoparticles. J Chem Phys 116:6755–6759CrossRefGoogle Scholar
  31. 31.
    Vasileva P, Donkova B, Karadjova I, Dushkin C (2011) Synthesis of starch-stabilized silver nanoparticles and their application as surface plasmon resonance based-sensor of hydrogen peroxide. Colloids Surf A 382:203–210CrossRefGoogle Scholar
  32. 32.
    Filippo E, Serra A, Manno D (2009) Poly(vinyl alcohol) capped silver nanoparticles as localized surface plasmon resonance-based hydrogen peroxide sensor. Sensors Actuators B 138:625–630CrossRefGoogle Scholar
  33. 33.
    Wang CC, Luconi MO, Masi AN, Fernandez LP (2009) Derivatized silver nanoparticles as sensor for ultra-trace nitrate determination based on light scattering phenomenon. Talanta 77:1238–1243CrossRefGoogle Scholar
  34. 34.
    Zheng J, Wu X, Wang M, Ran D, Xu W, Yang J (2008) Study on the interaction between silver nanoparticles and nucleic acids in the presence of cetyltrimethylammonium bromide and its analytical applications. Talanta 74:526–532CrossRefGoogle Scholar
  35. 35.
    Kang CY, Xi DL, Chen YY, Jiang ZL (2008) Determination of trace chlorine dioxide based on the plasmon resonance scattering of silver nanoparticles. Talanta 74:867–870CrossRefGoogle Scholar
  36. 36.
    Wu LP, Li YF, Hung CZ, Zhang Q (2006) Visual detection of Sudan dyes based on the plasmon resonance light scattering signals of silver nanoparticles. Anal Chem 78:5570–5577CrossRefGoogle Scholar
  37. 37.
    Wang HY, Li YF, Huang CZ (2007) Detection of ferulic acid based on the plasmon resonance light scattering of silver nanoparticles. Talanta 72:1698–1703CrossRefGoogle Scholar
  38. 38.
    Hormozi Nezhad MR, Tashkhourian J, Khodaveisi J, Khosi MR (2010) Simultaneous colorimetric determination of dopamine and ascorbic acid based on the surface plasmon resonance band of colloidal silver nanoparticles using artificial neural networks. Anal Methods 2:1263–1269CrossRefGoogle Scholar
  39. 39.
    Hormozi Nezhad MR, Tashkhourian J, Khodaveisic J (2010) Sensitive spectrophotometric detection of dopamine, levodopa and adrenaline using surface plasmon resonance band of silver nanoparticles. J Iran Chem Soc 7:S83–S91CrossRefGoogle Scholar
  40. 40.
    Tashkhourian J, Hormozi-Nezhad MR, Khodaveisi J (2011) Application of silver nanoparticles and principal component-artificial neural network models for simultaneous determination of levodopa and benserazide hydrochloride by a kinetic spectrophotometric method. Spectrochim Acta A 83:25–30Google Scholar
  41. 41.
    Wang H, Chen D, Wei Y, Chang Y, Zhao J (2011) A simple and sensitive assay of gallic acid based on localized surface plasmon resonance light scattering of silver nanoparticles through modified tollens process. Anal Sci 27:937–941CrossRefGoogle Scholar
  42. 42.
    Zhang F, Wu X, Zhan J (2011) Resonance light scattering technique for determination of polychlorinated biphenyls with silver nanoparticles. Luminescence 26:656–661CrossRefGoogle Scholar
  43. 43.
    Zandi-Atashbar N, Hemateenejad B, Akhond M (2011) Determination of amylose in Iranian rice by multivariate calibration of the surface plasmon resonance spectra of silver nanoparticles. Analyst 136:1760–1766CrossRefGoogle Scholar
  44. 44.
    Wang H, Chen D, Wei Y, Yu L, Zhang P, Zhao J (2011) A localized surface plasmon resonance light scattering-based sensing of hydroquinone via the formed silver nanoparticles in system. Spectrochim Acta A 79:2012–2016CrossRefGoogle Scholar
  45. 45.
    Bera RK, Anoop A, Raj CR (2011) Enzyme-free colorimetric assay of serum uric acid. Chem Commun 47:11498–11500CrossRefGoogle Scholar
  46. 46.
    Sharma VK, Yngard RA, Lin Y (2009) Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interf Sci 145:83–96CrossRefGoogle Scholar
  47. 47.
    Acheson RW (1976) An introduction to the chemistry of heterocyclic compounds, 3rd edn. John Wiley & Sons, New York, pp 420–421Google Scholar
  48. 48.
    Struck WA, Elving PJ (1965) Electrolytic oxidation of uric acid: products and mechanism. Biochemistry 4:1343–1353CrossRefGoogle Scholar
  49. 49.
    Poje M, Sokoloć-Mravić P (1988) The mechanism for the conversion of uric acid into allantoin and dehydro-allantoin: a new look at an old problem. Tetrahedron 42:747–751CrossRefGoogle Scholar
  50. 50.
    Kelsall RW, Hamley IW, Geoghegan M (2005) Nanoscale science and technology. John Wiley & Sons, Chichester, pp 45–46CrossRefGoogle Scholar
  51. 51.
    Simic MG, Jovanovic SV (1989) Antioxidation mechanism of uric acid. J Am Chem Soc 111:5778–5782CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Department of Analytical Chemistry, Faculty of ChemistryUniversity of TabrizTabrizIran

Personalised recommendations