Microchimica Acta

, Volume 178, Issue 3–4, pp 301–307 | Cite as

Comparative SPR study on the effect of nanomaterials on the biological activity of adsorbed proteins

  • Qiaohan Mei
  • Xiaorong Ding
  • Yangyang Chen
  • Jiang Hong
  • Kwangnak Koh
  • Jaebeom Lee
  • Hongxia Chen
  • Yongmei Yin
Original Paper


Bioactivity of proteins is evaluated to test the adverse effects of nanoparticles interjected into biological systems. Surface plasmon resonance (SPR) spectroscopy detects binding affinity that is normally related to biological activity. Utilizing SPR spectroscopy, a concise testing matrix is established by investigating the adsorption level of bovine serum albumin (BSA) and anti-BSA on the surface covered with 11-mercaptoundecanoic acid (MUA); magnetic nanoparticles (MNPs) and single-walled carbon nanotubes (SWCNTs), respectively. The immunoactivity of BSA on MNPs and SWCNT decreased by 18 % and 5 %, respectively, compared to that on the gold film modified with MUA. This indicates that MNPs cause a considerable loss of biological activity of adsorbed protein. This effect can be utilized for practical applications on detailed biophysical research and nanotoxicity studies.


Schematic diagram of Ab-Ag interaction on MNPs confined Au surface (left) and SPR study on the immunoactivity of BSA adsorbed on MNPs (right).


Surface plasmon resonance Magnetic nanoparticles BSA Bioactivity of proteins 



This work is supported by the National Natural Science Foundation of China (Grant Nos. 31100560, 81172503), Innovation Program of the Shanghai Municipal Education Commission (11YZ07) and by SRF for ROCS, SEM and the Leading Academic Discipline Project of Shanghai Municipal Education Commission (J50108).


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Qiaohan Mei
    • 1
  • Xiaorong Ding
    • 2
  • Yangyang Chen
    • 1
  • Jiang Hong
    • 1
  • Kwangnak Koh
    • 3
  • Jaebeom Lee
    • 3
  • Hongxia Chen
    • 1
  • Yongmei Yin
    • 2
  1. 1.Laboratory of Biosensing Technology, School of Life SciencesShanghai UniversityShanghaiChina
  2. 2.Department of Oncologythe First Affiliated Hospital of Nanjing Medical UniversityNanjingChina
  3. 3.Department of Nanomedical EngineeringPusan National UniversityMiryangRepublic of Korea

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