Comparative SPR study on the effect of nanomaterials on the biological activity of adsorbed proteins
Bioactivity of proteins is evaluated to test the adverse effects of nanoparticles interjected into biological systems. Surface plasmon resonance (SPR) spectroscopy detects binding affinity that is normally related to biological activity. Utilizing SPR spectroscopy, a concise testing matrix is established by investigating the adsorption level of bovine serum albumin (BSA) and anti-BSA on the surface covered with 11-mercaptoundecanoic acid (MUA); magnetic nanoparticles (MNPs) and single-walled carbon nanotubes (SWCNTs), respectively. The immunoactivity of BSA on MNPs and SWCNT decreased by 18 % and 5 %, respectively, compared to that on the gold film modified with MUA. This indicates that MNPs cause a considerable loss of biological activity of adsorbed protein. This effect can be utilized for practical applications on detailed biophysical research and nanotoxicity studies.
KeywordsSurface plasmon resonance Magnetic nanoparticles BSA Bioactivity of proteins
This work is supported by the National Natural Science Foundation of China (Grant Nos. 31100560, 81172503), Innovation Program of the Shanghai Municipal Education Commission (11YZ07) and by SRF for ROCS, SEM and the Leading Academic Discipline Project of Shanghai Municipal Education Commission (J50108).
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