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Microchimica Acta

, Volume 178, Issue 1–2, pp 229–236 | Cite as

Silver–gold core-shell nanoparticles containing methylene blue as SERS labels for probing and imaging of live cells

  • Xi Guo
  • Zhouyi GuoEmail author
  • Ying Jin
  • Zhiming Liu
  • Wen Zhang
  • Deqiu Huang
Original Paper

Abstract

We report on silver–gold core-shell nanostructures that contain Methylene Blue (MB) at the gold–silver interface. They can be used as reporter molecules in surface-enhanced Raman scattering (SERS) labels. The labels are stable and have strong SERS activity. TEM imaging revealed that these nanoparticles display bright and dark stripe structures. In addition, these labels can act as probes that can be detected and imaged through the specific Raman signatures of the reporters. We show that such SERS probes can identify cellular structures due to enhanced Raman spectra of intrinsic cellular molecules measured in the local optical fields of the core-shell nanostructures. They also provide structural information on the cellular environment as demonstrated for these nanoparticles as new SERS-active and biocompatible substrates for imaging of live cells.

Figure

The synthesis of MB embedded Ag/Au CS NPs ,and the results of these NPs were used in probing and imaging live cells as SERS labels

Keywords

Surface-enhanced Raman scattering Ag/Au core-shell nanoparticles A549 cell SERS imaging 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No.60778047), the Natural Science Foundation of Guangdong Province of China (Grant No. 9251063101000009), Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No.20114407110001),the cooperation project in industry, education and research of Guangdong province and Ministry of Education of P.R.China (Grant No. 2011A090200011) and the Key Science and Technology Project of Guangzhou City of China (Grant No. 2008Z1-D391).

Supplementary material

604_2012_829_MOESM1_ESM.doc (46 kb)
Table S1 (DOC 46 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Xi Guo
    • 1
  • Zhouyi Guo
    • 1
    Email author
  • Ying Jin
    • 1
  • Zhiming Liu
    • 1
  • Wen Zhang
    • 1
  • Deqiu Huang
    • 1
  1. 1.MOE Key Laboratory of Laser Life Science & Laboratory of Photonic Chinese Medicine, College of BiophotonicsSouth China Normal UniversityGuangzhouChina

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