Microchimica Acta

, Volume 177, Issue 1–2, pp 39–45 | Cite as

Non-enzymatic amperometric sensor for hydrogen peroxide based on a biocomposite made from chitosan, hemoglobin, and silver nanoparticles

  • Li TianEmail author
  • Yongjia Feng
  • Yanjuan Qi
  • Binbin Wang
  • Yinran Chen
  • Xueyan Fu
Original Paper


We report on a novel non-enzymatic sensor for hydrogen peroxide (HP) that is based on a biocomposite made up from chitosan (CS), hemoglobin (Hb), and silver nanoparticles (AgNPs). The AgNPs were prepared in the presence of CS and glucose in an ultrasonic bath, and CS is found to act as a stabilizing agent. They were then combined with Hb and CS to construct a carbon paste biosensor. The resulting electrode gave a well-defined redox couple for Hb, with a formal potential of about −0.17 V (vs. SCE) at pH 6.86 and exhibited a remarkable electrocatalytic activity for the reduction of HP. The sensor was used to detect HP by flow injection analysis, and a linear response is obtained in the 0.08 to 250 μM concentration range. The detection limit is 0.05 μM (at S/N = 3). These characteristics, along with its long-term stability make the sensor highly promising for the amperometric determination of HP.


(A) FIA it graphs of the different concentrations of H2O2 at CS/Hb/AgNP/CPE in the PBS (pH 6.86). Applied potential: −0.4 V. (1) 0.8 × 10−6 mol L−1, (2) 2.4 × 10−6 mol L−1, (3) 4 × 10−6 mol L−1 (B) Plot of catalytic peak currents vs. the concentration of H2O2.


Hydrogen peroxide Silver nanoparticles Hemoglobin Chitosan 



The authors acknowledge gratefully the financial support of the program of Science and Technology of department of education of Jilin Province and Changchun Normal University Natural Science Foundation.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Li Tian
    • 1
    Email author
  • Yongjia Feng
    • 2
  • Yanjuan Qi
    • 1
  • Binbin Wang
    • 1
  • Yinran Chen
    • 1
  • Xueyan Fu
    • 1
  1. 1.College of ChemistryChangchun Normal UniversityChangchunPeople’s Republic of China
  2. 2.Department of ChemistryYanbian UniversityYanjiPeople’s Republic of China

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