Microchimica Acta

, 174:191 | Cite as

Preparation and characterization of molecularly imprinted microspheres for selective extraction of trace melamine from milk samples

  • Bin Wang
  • Yuzhen Wang
  • Hong Yang
  • Junqing Wang
  • Anping Deng
Original Paper
First Online:
Received:
Accepted:

Abstract

We describe molecularly imprinted microspheres (MIMs) for the selective extraction of melamine from milk. The MIMs were made from melamine as the template molecule, methacrylic acid as the functional monomer, and ethylene glycol dimethacrylate as the linking agent. The MIMs were synthesized by suspension polymerization and characterized by rebinding experiments. They displayed high adsorption capacity, fast rebinding kinetics, and highly specific rebinding of melamine. The imprinting factor is 4.1. Scatchard analysis revealed a one-type rebinding behavior, the dissociation constant and maximum rebinding capacity being 37.59 g L−1 and 30.85 μmol g−1, respectively. The MIMs exhibited a 25% cross-reactivity towards atrazine, but less than 3.0% towards prometryn, clenbuterol and metronidazole. In addition, a MIM-based solid phase extraction (MISPE) column for melamine was prepared by packing MIMs into a common SPE cartridge. The MISPE extraction gave recoveries of 89.8 to 100.6% of melamine, with relative standard deviations of 5.9 to 7.5%. There was no significant loss of rebinding capacity after more than 60 repeated uses, thus demonstrating the high stability of the MISPE column. The MSPE column also was applied to the extraction of melamine from spiked liquid and powdered milk with satisfying accuracy and precision.

Figure

The melamine molecularly imprinted microsphere (MIMs) prepared by suspension polymerization displayed high adsorption capacity quick rebinding kinetic and highly specific rebinding. The MIMs-based solid phase extraction (MISPE) column was prepared by packing MIMs into a common SPE cartridge. The recoveries of MISPE for extracting melamine from milk samples were 76.26–90.95%.

Keywords

Molecular imprinted microspheres (MIMs) Melamine Extraction Milk sample 
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Notes

Acknowledgements

The authors thank the National Natural Science Foundation of China (NSFC) for financial support of this study (contact no. 20675054 and no. 20835003).

Supplementary material

604_2011_613_MOESM1_ESM.doc (184 kb)
ESM 1 (DOC 183 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Bin Wang
    • 1
  • Yuzhen Wang
    • 1
  • Hong Yang
    • 2
  • Junqing Wang
    • 1
  • Anping Deng
    • 1
    • 3
  1. 1.College of ChemistrySichuan UniversityChengduChina
  2. 2.College of PharmacySoochow UniversitySuzhouChina
  3. 3.College of Chemistry, Chemical Engineering and Materials ScienceSoochow UniversitySuzhouChina

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