Microchimica Acta

, Volume 174, Issue 1–2, pp 31–39 | Cite as

Electrochemical determination of NADH using a glassy carbon electrode modified with Fe3O4 nanoparticles and poly-2,6-pyridinedicarboxylic acid, and its application to the determination of antioxidant capacity

  • Lin Cui
  • Shiyun Ai
  • Kun Shang
  • Xiaomeng Meng
  • Chengcheng Wang
Original Paper
First Online:
Received:
Accepted:

Abstract

We have prepared a glassy carbon electrode modified with poly-2,6-pyridinedicarboxylic acid and with magnetic Fe3O4 nanoparticles. This modification enhances the effective surface area and the electrocatalytic oxidation of nicotinamide adenine dinucleotide (NADH) in addition to providing positively charged groups for electrostatic assembly of the phosphate group of NADH. The modified electrode responds linearly to NADH in the range from 5 × 10−8 to 2.5 × 10−5 M and gives a lower detection limit of 1 × 10−8 M. It displays satisfactory selectivity and reproducibility. The sensor was applied to rapid screening of plant extracts for their antioxidant properties.

Figure

Poly-2,6-pyridinedicarboxylic acid (PDC) was fabricated by electropolymerizing 2,6-pyridinedicarboxylic acid with cyclic voltammetry (CV) on the glassy carbon electrode (GCE) surface. The magnetic Fe3O4 nanoparticles treated with aminopropyltriethoxysilane (APTS) modified on the PDC/GCE to form APTS-Fe3O4/PDC composite film. The APTS-Fe3O4/PDC film had enhanced the effective electrode surface area and provided positively charged groups for electrostatic assembly of phosphate group of NADH.

Keywords

APTS-Fe3O4 nanoparticles Poly-2,6-pyridinedicarboxylic acid NADH Determination Electrochemistry 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No.201075078) and the Natural Science Foundation of Shandong province, China (ZR2010BM05).

Supplementary material

604_2011_594_MOESM1_ESM.doc (422 kb)
ESM 1 (DOC 422 kb)

References

  1. 1.
    Katakis I, Domínguez E (1997) Catalytic electrooxidation of NADH for dehydrogenase amperometric biosensors mikrochim. Acta 126:11–32Google Scholar
  2. 2.
    Wu Q, Maskus M, Pariente F, Tobalina F, Fernández V, Lorenzo E, Abruña H (1996) Electrocatalytic oxidation of NADH at glassy carbon electrodes modified with transition metal complexes containing 1, 10-phenanthroline-5, 6-dione ligands. Anal Chem 68:3688–3696CrossRefGoogle Scholar
  3. 3.
    Popescu IC, Domínguez E, Narváez A, Pavlov V, Katakis I (1999) Electrocatalytic oxidation of NADH at graphite electrodes modified with osmium phenanthrolinedione. J Electroanal Chem 464:208–214CrossRefGoogle Scholar
  4. 4.
    Somasundrum M, Hall J, Bannister J (1994) Amperometric NADH determination via both direct and mediated electron transfer by NADH oxidase from Thermus aquaticus YT-1. Anal Chim Acta 295:47–57CrossRefGoogle Scholar
  5. 5.
    Shan C, Yang H, Han D, Zhang Q, Ivaska A, Niu L (2010) Electrochemical determination of NADH and ethanol based on ionic liquid-functionalized graphene. Biosens Bioelectron 25:1504–1508CrossRefGoogle Scholar
  6. 6.
    Toh C, Bartlett P, Mano N, Aussenac F, Kuhn A, Dufourc E (2003) The effect of calcium ions on the electrocatalytic oxidation of NADH by poly (aniline)-poly (vinylsulfonate) and poly (aniline)-poly (styrenesulfonate) modified electrodes. Phys Chem Chem Phys 5:588–593CrossRefGoogle Scholar
  7. 7.
    Bartlett P, Simon E (2000) Poly (aniline)-poly (acrylate) composite films as modified electrodes for the oxidation of NADH. Phys Chem Chem Phys 2:2599–2606CrossRefGoogle Scholar
  8. 8.
    Lobo M, Miranda A, López-Fonseca J, Tuñón P (1996) Electrocatalytic detection of nicotinamide coenzymes by poly (o-aminophenol)-and poly (o-phenylenediamine)-modified carbon paste electrodes. Anal Chim Acta 325:33–42CrossRefGoogle Scholar
  9. 9.
    Torstensson A, Gorton L (1981) Catalytic oxidation of NADH by surface-modified graphite electrodes. J Electroanal Chem 130:199–207Google Scholar
  10. 10.
    Kulys J (1981) Development of new analytical systems based on biocatalysers. Anal Lett 14:377Google Scholar
  11. 11.
    Ni F, Feng H, Gorton L, Cotton T (1990) Electrochemical and SERS studies of chemically modified electrodes: nile blue A, a mediator for NADH oxidation. Langmuir 6:66–73CrossRefGoogle Scholar
  12. 12.
    Gorton L, Torstensson A, Jaegfeldt H, Johansson G (1984) Electrocatalytic oxidation of reduced nicotinamide coenzymes by graphite electrodes modified with an adsorbed phenoxazinium salt. Meldola Blue. J Electroanal Chem 161:103–120CrossRefGoogle Scholar
  13. 13.
    Persson B, Gorton L (1990) A comparative study of some 3, 7-diaminophenoxazine derivatives and related compounds for electrocatalytic oxidation of NADH. J Electroanal Chem 292:115–138CrossRefGoogle Scholar
  14. 14.
    Patolsky F, Lieber C (2005) Nanowire nanosensors. Mater Today 8:20–28CrossRefGoogle Scholar
  15. 15.
    Grimes C, Singh R, Dickey E, Varghese O (2001) Metal-oxide films with magnetically-modulated nanoporous architectures. J Mater Res 16:1686–1693CrossRefGoogle Scholar
  16. 16.
    Ramírez F, Tarancón A, Casals O, Rodríguez J, Romano-Rodríguez A, Morante J, Barth S, Mathur S, Choi T, Poulikakos D (2006) Fabrication and electrical characterization of circuits based on individual tin oxide nanowires. Nanotechnol 17:5577–5583CrossRefGoogle Scholar
  17. 17.
    Mathur S, Sivakov V, Shen H, Barth S, Cavelius C, Nilsson A, Kuhn P (2006) Nanostructured films of iron, tin and titanium oxides by chemical vapor deposition. Thin Solid Films 502:88–93CrossRefGoogle Scholar
  18. 18.
    Cao D, Hu N (2006) Direct electron transfer between hemoglobin and pyrolytic graphite electrodes enhanced by Fe3O4 nanoparticles in their layer-by-layer self-assembly films. Biophys Chem 121:209–217CrossRefGoogle Scholar
  19. 19.
    Dresco P, Zaitsev V, Gambino R, Chu B (1999) Preparation and properties of magnetite and polymer magnetite nanoparticles. Langmuir 15:1945–1951CrossRefGoogle Scholar
  20. 20.
    Mehta R, Upadhyay R, Charles S, Ramchand C (1997) Direct binding of protein to magnetic particles. Biotechnol Tech 11:493–496CrossRefGoogle Scholar
  21. 21.
    Koneracká M, Kopčanský P, Antalík M, Timko M, Ramchand C, Lobo D, Mehta R, Upadhyay R (1999) Immobilization of proteins and enzymes to fine magnetic particles. J Magn Magn Mater 201:427–430CrossRefGoogle Scholar
  22. 22.
    Liu X, Ma Z, Xing J, Liu H (2004) Preparation and characterization of amino-silane modified superparamagnetic silica nanospheres. J Magn Magn Mater 270:1–6CrossRefGoogle Scholar
  23. 23.
    Xie W, Ma N (2009) Immobilized lipase on Fe3O4 nanoparticles as biocatalyst for biodiesel production. Energy Fuels 23:1347–1353CrossRefGoogle Scholar
  24. 24.
    Yang J, Yang T, Feng Y, Jiao K (2007) A DNA electrochemical sensor based on nanogold-modified poly-2, 6-pyridinedicarboxylic acid film and detection of PAT gene fragment. Anal Biochem 365:24–30CrossRefGoogle Scholar
  25. 25.
    Mello L, Hernandez S, Marrazza G, Mascini M, Kubota L (2006) Investigations of the antioxidant properties of plant extracts using a DNA-electrochemical biosensor. Biosens Bioelectron 21:1374–1382CrossRefGoogle Scholar
  26. 26.
    Labuda J, Bučková M, Jantová S, Štepánek I, Surugiu I, Danielsson B, Mascini M (2000) Modified screen-printed electrodes for the investigation of the interaction of non-electroactive quinazoline derivatives with DNA. Fres J Anal Chem 367:364–368CrossRefGoogle Scholar
  27. 27.
    Mascini M, Palchetti I, Marrazza G (2001) DNA electrochemical biosensors. Fres J Anal Chem 369:15–22CrossRefGoogle Scholar
  28. 28.
    Oliveira-Brett A, Diculescu V, Piedade J (2002) Electrochemical oxidation mechanism of guanine and adenine using a glassy carbon microelectrode. Bioelectrochemistry 55:61–62CrossRefGoogle Scholar
  29. 29.
    Erdem A, Ozsoz M (2002) Electrochemical DNA biosensors based on DNA-drug interactions. Electroanalysis 14:965–974CrossRefGoogle Scholar
  30. 30.
    Labuda J, BučkováM HL, Žiaková A, Brandšteterová E, Mattusch J, Wennrich R (2002) Detection of antioxidative activity of plant extracts at the DNA-modified screen-printed electrode. Sensors 2:1–10CrossRefGoogle Scholar
  31. 31.
    Labuda J, BučkováM HL, Šilhár S, Štepánek I (2003) Evaluation of the redox properties and anti/pro-oxidant effects of selected flavonoids by means of a DNA-based electrochemical biosensor. Anal Bioanal Chem 376:168–173Google Scholar
  32. 32.
    Brett A, Piedade J, Serrano S (2000) Electrochemical oxidation of 8-oxoguanine. Electroanalysis 12:969–973CrossRefGoogle Scholar
  33. 33.
    Ningappa M, Dinesha R, Srinivas L (2008) Antioxidant and free radical scavenging activities of polyphenol-enriched curry leaf (Murraya koenigii L.) extracts. Food Chem 106:720–728CrossRefGoogle Scholar
  34. 34.
    Kim D, Zhang Y, Kehr J, Klason T, Bjelke B, Muhammed M (2001) Characterization and MRI study of surfactant-coated superparamagnetic nanoparticles administered into the rat brain. J Magn Magn Mater 225:256–261CrossRefGoogle Scholar
  35. 35.
    Matsuno R, Yamamoto K, Otsuka H, Takahara A (2003) Polystyrene-grafted magnetite nanoparticles prepared through surface-initiated nitroxyl-mediated radical polymerization. Chem Mater 15:3–5CrossRefGoogle Scholar
  36. 36.
    Zare H, Nasirizadeh N, Mazloum-Ardakani M, Namazian M (2006) Electrochemical properties and electrocatalytic activity of hematoxylin modified carbon paste electrode toward the oxidation of reduced nicotinamide adenine dinucleotide (NADH). Sens Actuators B: Chem 120:288–294CrossRefGoogle Scholar
  37. 37.
    Laviron E (1974) Adsorption, autoinhibition and autocatalysis in polarography and in linear potential sweep voltammetry. J Electroanal Chem 52:355–393CrossRefGoogle Scholar
  38. 38.
    Golabi S, Irannejad L (2005) Preparation and electrochemical study of fisetin modified glassy carbon electrode. Application to the determination of NADH and ascorbic acid. Electroanalysis 17:985–996CrossRefGoogle Scholar
  39. 39.
    Adams RN (1969) Electrochemistry at solid electrodes. Marcel Dekker, New YorkGoogle Scholar
  40. 40.
    Anson F (1964) Application of potentiostatic current integration to the study of the adsorption of cobalt (III)-(Ethylenedinitrilo (tetraacetate) on mercury electrodes. Anal Chem 36:932–934CrossRefGoogle Scholar
  41. 41.
    Katekawa E, Maximiano F, Rodrigues L, Delbem MF, Serrano S (1999) Electrochemical oxidation of NADH at a bare glassy carbon electrode in different supporting electrolytes. Anal Chim Acta 385:345–352CrossRefGoogle Scholar
  42. 42.
    Gurban A, Noguer T, Bala C, Rotariu L (2008) Improvement of NADH detection using Prussian blue modified screen-printed electrodes and different strategies of immobilisation. Sens Actuators B: Chem 128:536–544CrossRefGoogle Scholar
  43. 43.
    Golabi S, Zare H, Hamzehloo M (2002) Electrochemistry and electrocatalytic activity of pyrocatechol violet (PCV) film on a glassy carbon electrode towards the oxidation of reduced nicotinamide adenine dinucleotide (NADH). Electroanalysis 14:611–618CrossRefGoogle Scholar
  44. 44.
    Radoi A, Compagnone D, Devic E, Palleschi G (2007) Low potential detection of NADH with Prussian Blue bulk modified screen-printed electrodes and recombinant NADH oxidase from Thermus thermophilus. Sens Actuators B 121:501–506CrossRefGoogle Scholar
  45. 45.
    Meng L, Wu P, Chen G, Cai C, Sun Y, Yuan Z (2009) Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode. Biosens Bioelectron 24:1751–1756CrossRefGoogle Scholar
  46. 46.
    Gao Q, Sun M, Peng P, Qi HL, Zhang CX (2010) Electro-oxidativepolymerization of phenothiazine dyes into a multilayer-containing carbon nanotube on a glassy carbon electrode for the sensitive and low-potential detection of NADH. Microchim Acta 168:299–307CrossRefGoogle Scholar
  47. 47.
    Zhu L, Zhai J, Yang R, Tian C, Guo L (2007) Electrocatalytic oxidation of NADH with Meldola’s blue functionalized carbon nanotubes electrodes. Biosens Bioelectron 22:2768–2773CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Lin Cui
    • 1
  • Shiyun Ai
    • 1
  • Kun Shang
    • 1
  • Xiaomeng Meng
    • 1
  • Chengcheng Wang
    • 2
  1. 1.College of Chemistry and Material ScienceShandong Agricultural UniversityTaianPeople’s Republic of China
  2. 2.College of Chemistry and Chemical EngineeringNanjing UniversityNanjingPeople’s Republic of China

Personalised recommendations