Microchimica Acta

, Volume 174, Issue 1–2, pp 55–61 | Cite as

Hydrogen peroxide biosensor based on horseradish peroxidase immobilized on chitosan-wrapped NiFe2O4 nanoparticles

  • Liqiang LuoEmail author
  • Limei Zhu
  • Yanhong Xu
  • Liuyi Shen
  • Xia Wang
  • Yaping DingEmail author
  • Qiuxia Li
  • Dongmei Deng
Original Paper


A biosensor for hydrogen peroxide was constructed by immobilizing horseradish peroxidase on chitosan-wrapped NiFe2O4 nanoparticles on a glassy carbon electrode (GCE). The electron mediator carboxyferrocene was also immobilized on the surface of the GCE. UV–vis spectra, Fourier transform IR spectra, scanning electron microscopy, and electrochemical impedance spectra were acquired to characterize the biosensor. The experimental conditions were studied and optimized. The biosensor responds linearly to H2O2 in the range from 1.0 × 10−5 to 2.0 × 10−3 M and with a detection limit of 2.0 × 10−6 M (at S/N = 3).


A biosensor for hydrogen peroxide was constructed by immobilizing horseradish peroxidase on chitosan-wrapped NiFe2O4 nanoparticles on a glassy carbon electrode.


Chitosan NiFe2O4 nanoparticles Horseradish peroxidase Ferrocene carboxylic acid Biosensor 



This work is supported by the National Natural Science Foundation of China (No. 10804067, 20975066), Leading Academic Discipline Project of Shanghai Municipal Education Commission (J50102), and the State Key Laboratory of Electroanalytical Chemistry, China (No. 2009005).


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Liqiang Luo
    • 1
    Email author
  • Limei Zhu
    • 1
  • Yanhong Xu
    • 1
  • Liuyi Shen
    • 1
  • Xia Wang
    • 1
  • Yaping Ding
    • 1
    Email author
  • Qiuxia Li
    • 1
  • Dongmei Deng
    • 1
  1. 1.College of SciencesShanghai UniversityShanghaiPeople’s Republic of China

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