Microchimica Acta

, Volume 173, Issue 1–2, pp 189–197 | Cite as

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA

  • Dalibor Huska
  • Vojtech Adam
  • Petr Babula
  • Libuse Trnkova
  • Jaromir Hubalek
  • Josef Zehnalek
  • Ladislav Havel
  • Rene KizekEmail author
Original Paper


The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and poly-adenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.


Microfluidic robotic device coupled with electrochemical sensor field


Electrochemical detection Microfluidics Environmental stress Cadmium ions Paramagnetic particles 



The financial support from the grant NANOSEMED KAN208130801, INCHEMBIOL MSM0021622412 and REMEDTECH GACR 522/07/0692 is highly acknowledged. The authors wish to express their thanks to Jiri Baloun for excellent technical assistance.

Supplementary material

604_2011_545_MOESM1_ESM.doc (3 mb)
ESM 1 (DOC 3080 kb)


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Dalibor Huska
    • 1
    • 2
  • Vojtech Adam
    • 1
  • Petr Babula
    • 3
  • Libuse Trnkova
    • 4
  • Jaromir Hubalek
    • 5
  • Josef Zehnalek
    • 1
  • Ladislav Havel
    • 2
  • Rene Kizek
    • 1
    Email author
  1. 1.Department of Chemistry and BiochemistryMendel UniversityBrnoCzech Republic
  2. 2.Department of Plant BiologyMendel UniversityBrnoCzech Republic
  3. 3.Department of Natural Drugs, Faculty of PharmacyUniversity of Veterinary and Pharmaceutical SciencesBrnoCzech Republic
  4. 4.Department of Chemistry, Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  5. 5.Department of Microelectronics, Faculty of Electrical Engineering and CommunicationBrno University of TechnologyBrnoCzech Republic

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