The effect of colloidal solutions of gold nanoparticles on the performance of a glucose oxidase modified carbon electrode
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The aim of this work was to further evaluate the effect of colloidal solutions of gold nanoparticles (Au-NPs) on the performance of a carbon rod electrode modified with glucose oxidase. The amperometric response of the system at +0.3 V vs. Ag/AgCl was studied in the absence and in the presence of Au-NPs (6 nm and 13 nm in diameter) and in presence of N-methylphenazonium methyl sulfate (PMS) at pH 6.0. This study shows that the application of <0.60 nmol L−1 concentrations of Au-NPs increases the rate of mediated electron transfer, and this effect does not depend on the diameter of Au-NPs. The analytical signal in the presence 0.60 nmol L−1 of Au-NPs (13 nm) and 2 mmol L−1 of PMS linearly depends on the concentration of glucose in the range from 0.1 to 10 mmol L−1, the limit of detection is as low as 0.05 mmol L−1.
KeywordsCarbon rod electrode Electron transfer Gold nanoparticles Glucose oxidase Amperometry Mediator Biosensor
This work was financially supported by Lithuanian State Science and Studies Foundation project number S-6/2007 and COST program D43.
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