Microchimica Acta

, Volume 169, Issue 3–4, pp 361–365 | Cite as

Electrodeposition of silver nanoparticles on a zinc oxide film: improvement of amperometric sensing sensitivity and stability for hydrogen peroxide determination

  • Qi Wang
  • Jianbin ZhengEmail author
Original Paper


A strategy to fabricate a hydrogen peroxide (HP) sensor is developed by electrodepositing silver nanoparticles (Ag NPs) on a modified glassy carbon electrode (GCE) with a zinc oxide (ZnO) film. The Ag NPs/ZnO/GCE has been characterized by scanning electron microscopy, cyclic voltammetry, and chronoamperometry. It has been found that the Ag NPs synthesized in the presence of ZnO film provide an electrode with enhanced sensitivity and excellent stability. The sensitivity to HP is enhanced 3-fold by using Ag NPs/ZnO/GCE compared to Ag NPs/GCE. The HP sensor exhibits good linear behavior in the concentration range 2 µM to 5.5 mM for the quantitative analysis of HP with a detection limit of 0.42 µM (S/N = 3).


Modified electrode Non-enzymatic sensors Silver nanoparticles Zinc oxide Hydrogen peroxide 



This work is supported by the National Natural Science Foundation of China (No. 20875076).

Supplementary material

604_2010_356_MOESM1_ESM.doc (30 kb)
ESM 1 (DOC 30 kb)


  1. 1.
    Lei W, Duerkop A, Lin Z, Wu M, Wolfbeis OS (2003) Detection of hydrogen peroxide in river water via a microplate luminescence assay with time-resolved (“gated”) detection. Microchimi Acta 143:269CrossRefGoogle Scholar
  2. 2.
    Wang J, Lin Y, Chen L (1993) Organic-phase biosensors for monitoring phenol and hydrogen peroxide in pharmaceutical antibacterial products. Analyst 118:277CrossRefGoogle Scholar
  3. 3.
    Wang J (2008) Electrochemical glucose biosensors. Chem Rev 108:814CrossRefGoogle Scholar
  4. 4.
    Wang B, Li B, Wang Z, Xu G, Wang Q, Dong S (1999) Sol–gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium. Anal Chem 71:1935CrossRefGoogle Scholar
  5. 5.
    Che X, Yuan R, Chai Y, Ma L, Li W, Li J (2009) Hydrogen peroxide sensor based on horseradish peroxidase immobilized on an electrode modified with DNA-L-cysteine-gold-platinum nanoparticles in polypyrrole film. Microchim Acta 167:159CrossRefGoogle Scholar
  6. 6.
    Lai GS, Zhang HL, Han DY (2009) Amperometric hydrogen peroxide biosensor based on the immobilization of horseradish peroxidase by carbon-coated iron nanoparticles in combination with chitosan and cross-linking of glutaraldehyde. Microchim Acta 165:159CrossRefGoogle Scholar
  7. 7.
    Kang XH, Mai ZB, Zou XY, Cai PX, Mo JY (2007) A sensitive nonenzymatic glucose sensor in alkaline media with a copper nanocluster/multiwall carbon nanotube-modified glassy carbon electrode. Anal Biochem 363:143CrossRefGoogle Scholar
  8. 8.
    Luo F, Yin J, Gao F, Wang L (2009) A non-enzyme hydrogen peroxide sensor based on core/shell silica nanoparticles using synchronous fluorescence spectroscopy. Microchim Acta 165:23CrossRefGoogle Scholar
  9. 9.
    Li Y, Liu X, Zeng X, Liu Y, Liu X, Wei W, Luo S (2009) Nonenzymatic hydrogen peroxide sensor based on a Prussian Blue-modified carbon ionic liquid electrode. Microchim Acta 165:393CrossRefGoogle Scholar
  10. 10.
    Guascito MR, Filippo E, Malitesta C, Manno D, Serra A, Turco A (2008) A new amperometric nanostructured sensor for the analytical determination of hydrogen peroxide. Biosens Bioelectron 24:1057CrossRefGoogle Scholar
  11. 11.
    Xiao F, Zhao F, Zhang Y, Gai G, Zeng B (2009) Ultrasonic electrodeposition of gold-platinum alloy nanoparticles on ionic liquid-chitosan composite film and their application in fabricating nonenzyme hydrogen peroxide sensors. J Phys Chem C 113:849CrossRefGoogle Scholar
  12. 12.
    Welch CM, Banks CE, Simm AO, Compton RG (2005) Silver nanoparticle assemblies supported on glassy-carbon electrodes for the electro-analytical detection of hydrogen peroxide. Anal Bioanal Chem 382:12CrossRefGoogle Scholar
  13. 13.
    Cui K, Song Y, Yao Y, Huang Z, Wang L (2008) A novel hydrogen peroxide sensor based on Ag nanoparticles electrodeposited on DNA-networks modified glassy carbon electrode. Electrochem Commun 10:663CrossRefGoogle Scholar
  14. 14.
    Flatgen G, Wasle S, Lübke M, Eickes C, Radhakrishnan G, Doblhofer K, Ertl G (1999) Autocatalytic mechanism of H2O2 reduction on Ag electrodes in acidic electrolyte: experiments and simulations. Electrochim Acta 44:4499CrossRefGoogle Scholar
  15. 15.
    Safavi A, Maleki N, Farjamia E (2009) Electrodeposited silver nanoparticles on carbon ionic liquid electrode for electrocatalytic sensing of hydrogen peroxide. Electroanalysis 21:1533CrossRefGoogle Scholar
  16. 16.
    Andersson M, Pedersen JS, Palmqvist AEC (2005) Silver nanoparticle formation in microemulsions acting both as template and reducing agent. Langmuir 21:11387CrossRefGoogle Scholar
  17. 17.
    El-Deab MS, Sotomura T, Ohsaka T (2006) Oxygen reduction at Au nanoparticles electrodeposited on different carbon substrates. Electrochim Acta 52:1792CrossRefGoogle Scholar
  18. 18.
    El-Deab MS, Ohsaka T (2007) Electrocatalysis by design: effect of the loading level of Au nanoparticles–MnOx nanoparticles binary catalysts on the electrochemical reduction of molecular oxygen. Electrochim Acta 52:2166CrossRefGoogle Scholar
  19. 19.
    Penner RM (2002) Mesoscopic metal particles and wires by electrodeposition. J Phys Chem B 106:3339CrossRefGoogle Scholar
  20. 20.
    Beydoun D, Amal R, Low G, McEvoy S (1999) Role of nanoparticles in photocatalysis. J Nanopart Res 1:439CrossRefGoogle Scholar
  21. 21.
    Daniel MC, Astruc D (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104:293CrossRefGoogle Scholar
  22. 22.
    Li D, Haneda HJ (2003) Morphologies of zinc oxide particles and their effects on photocatalysis. Chemosphere 51:129CrossRefGoogle Scholar
  23. 23.
    Comini E, Faglia G, Sberveglieri G, Pan ZW, Wang ZL (2002) Influence of Ag particle size on ethanol sensing of SnO1.8: Ag nanoparticle films: a method to develop parts per billion level gas sensors. Appl Phys Lett 81:1869CrossRefGoogle Scholar
  24. 24.
    Arnold MS, Avouris P, Pan ZW, Wang ZL (2002) Field-effect transistors based on single semiconducting oxide nanobelts. J Phys Chem B 107:659CrossRefGoogle Scholar
  25. 25.
    Kumar SA, Cheng HW, Chen SM, Wang SF (2010) Preparation and characterization of copper nanoparticles/zinc oxide composite modified electrode and its application to glucose sensing. Mater Sci Eng C 30:86CrossRefGoogle Scholar
  26. 26.
    Pal U, Segura GC, Corona OZ (2003) Preparation of Ge/ZnO nanocomposites by radio frequency alternate sputtering. Sol Energ Mater Sol Cells 76:305CrossRefGoogle Scholar
  27. 27.
    Xu J, Yang H, Fu W, Fan W, Zhu Q, Li M, Zou G (2008) Synthesis and characterization of nickel coated by zinc oxide: bifunctional magnetic-optical nanocomposites. J Alloys Compd 458:119CrossRefGoogle Scholar
  28. 28.
    Zhao W, Wang H, Qin X, Wang X, Zhao Z, Miao Z, Chen L, Shan M, Fang Y, Chen Q (2009) A novel nonenzymatic hydrogen peroxide sensor based on multi-wall carbon nanotube/silver nanoparticle nanohybrids modified gold electrode. Talanta 80:1029CrossRefGoogle Scholar
  29. 29.
    Tao WY, Pan DW, Liu YJ, Nie LH, Yao SZ (2005) An amperometric hydrogen peroxide sensor based on immobilization of hemoglobin in poly (o-aminophenol) film at iron–cobalt hexacyanoferrate-modified gold electrode. Anal Biochem 338:332CrossRefGoogle Scholar
  30. 30.
    Wang L, Wang EK (2004) A novel hydrogen peroxide sensor based on horseradish peroxidase immobilized on colloidal Au modified ITO electrode. Electrochem Commun 6:225CrossRefGoogle Scholar
  31. 31.
    Lei C, Hua S, Shen G, Yu R (2003) Immobilization of horseradish peroxidase to a nano-Au monolayer modified chitosan-entrapped carbon paste electrode for the detection of hydrogen peroxide. Talanta 59:981CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Electroanalytical ChemistryNorthwest UniversityXi´anPeople’s Republic of China
  2. 2.School of ScienceXidian UniversityXi´anPeople’s Republic of China

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