Microchimica Acta

, Volume 168, Issue 3–4, pp 317–324 | Cite as

Dispersive liquid-liquid microextraction followed by spectrofluorimetry as a simple and accurate technique for determination of thiamine (vitamin B1)

  • Mohsen Zeeb
  • Mohammad Reza Ganjali
  • Parviz Norouzi
Original Paper


Dispersive liquid-liquid microextraction (DLLME) combined with spectrofluorimetry was applied to the extraction, pre-concentration and analysis of thiamine (vitamin B1). The procedure is based on (a) the oxidation of thiamine with ferricyanide to form fluorescent thiochrome (TC), (b) the trapping of TC into a microextraction solvent, and (c) spectrofluorometric determination. Microextraction solvent and disperser solvent are directly injected into an aqueous solution containing TC. After centrifuging, phase separation is performed by sedimenting the fine droplets of the microextraction solvent on the bottom of a test tube. The settled phase is transferred into a fluorometer for the determination of thiamine at excitation/emission wavelengths of 375/438 nm. Under the optimized experimental conditions, the method provides a linear dynamic range of 0.2–100 ng mL−1, a detection limit of 0.06 ng mL−1, and a relative standard deviation of 3.0%. The method was successfully applied to pharmaceutical formulations and human urine. The results were validated by recovery test and by comparison with other methods, and were found to be highly satisfactory.


Thiamine Spectrofluorimetry Dispersive liquid-liquid microextraction Pharmaceutical Urine 



Support of this investigation by the Research Council of University of Tehran through Grant is gratefully acknowledged. We also thank Miss Saeideh Noushini for the help to do the voltammetric method as a reference method.

Supplementary material

604_2009_282_MOESM1_ESM.doc (73 kb)
ESM 1 (DOC 73 kb)


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Mohsen Zeeb
    • 1
  • Mohammad Reza Ganjali
    • 1
  • Parviz Norouzi
    • 2
  1. 1.Center of Excellence in Electrochemistry, Faculty of ChemistryUniversity of TehranTehranIran
  2. 2.Endocrinology & Metabolism Research CenterTehran University of Medical SciencesTehranIran

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